HPLC同时测定复方黄连素片中3种成分的含量  

Simultaneous Determination of Peoniflorin,Costunolide,Dehydrocostus Lactone in Compound Berberine Tablets by HPLC

作　　者：刘晓哲 张东方 

机构地区：[1]河南省南阳市食品药品检验所,河南南阳473059

出　　处：《中国实验方剂学杂志》2014年第1期70-73,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技厅重点科技攻关项目(112102210030)

摘　　要：目的：同时测定复方黄连素片中芍药苷、木香烃内酯、去氢木香内酯3种成分的含量。，方法：采用高效液相色谱法，C18柱，以乙腈（A）-0．2％的磷酸溶液（B）为流动相进行梯度洗脱，流速1．0mL·min^-1，检测波长225nm，柱温30℃。结果：芍药苷在0．1809～3．618μg线性关系良好（r=0．9998），平均加样回收率为99．0％，RSD0．94％；木香烃内酯在0．12985～2．597μg线性关系良好（r=0．9997），平均加样回收率为98．4％，RSD1．62％；去氢木香内酯在0．1208～2．416μg线性关系良好（r=0．9996），平均加样回收率为97．9％，RSD1．21％。结论：该方法简便、准确，可用于测定复方黄连素片的含量。Objective： To establish a method for simultaneous determining peoniflorin, costunolide and dehydrocostus lactone in compound berberine tablets. Method： Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.2% phosphoric acid solution at a flow rate of 1.0 mL -min 1 , detection wavelength was 225 nm, and column temperature of 30 ℃. Result： The linear ranges of peoniflorin, costunolide, and dehydroeostus lactone fell within the ranges of 0. 180 9-3. 618, 0. 129 85-2. 597, 0. 120 8-2. 416 ~g, respectively. The three components showed good linear correlations （r = 0. 999 8, 0. 999 7, 0. 999 6）. The recoveries were 99.0% for peoniflorin, 98.4% for costunolide, 97.9% for dehydrocostus lactone, respectively. The relative standard deviations were 0.94%, 1.62% and 1.21%. Conclusion ： The method is simple and accurate. It can be used for quality control of compound berberine tablets.

关 键 词：液相色谱 复方黄连素片 芍药苷 木香烃内酯 去氢木香内酯 

分 类 号：R284.1[医药卫生—中药学]