瓜蒌皮药材的HPLC指纹图谱  

HPLC Fingerprint of Pericarpium trichosanthis

作　　者：史国玉[1,2,3] 刘伟[1,2] 耿岩玲[1,2] 刘建华[1,2] 王晓[1,2] 周凤琴[4] 

机构地区：[1]山东省科学院中药过程控制研究中心 [2]山东省分析测试中心,济南250014 [3]山东医学高等专科学校,济南250002 [4]山东中医药大学,济南250355

出　　处：《中国实验方剂学杂志》2014年第1期73-76,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："十二五"国家科技支撑计划课题(2011BAI06B06)

摘　　要：目的:建立瓜蒌皮药材的HPLC指纹图谱。方法:采用Agilent TC-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.2%冰乙酸为流动相进行梯度洗脱,检测波长260 nm,流速0.8 mL·min-1,柱温25℃,进样20μL。结果:建立了瓜蒌皮药材的HPLC指纹图谱,方法学考察结果良好,确立了13个共有峰,其中5个共有峰得到确认,10批瓜蒌皮样品指纹图谱的相似度均>0.9。结论:该方法稳定性、重复性好,建立的指纹图谱可为瓜蒌皮的质量评价提供依据。Objective： To establish HPLC fingerprint of P. trichosanthis. Method： The HPLC method was used on an Agilent TC-Cls （4.6 mm ×250 mm, 5 μm） chromatographic column with acetonitrile-0. 2% ice acetic acid as mobile phase in gradient elution at the detection wavelength of 260 nm with the flow rate of 0.8 mL . min^-1, and the column temperature was 25 ℃ , the sample injection was 20 μL. Result： The HPLC fingerprint of P. trichosanthis was established. The methodological investigation result was good. 13 common peaks were existed in the fingerprint and five common peaks were confirmed. The similarities of 10 batches of P. trichosanthis are greater than 0.9. Conclusion： The method is stable, and the established fingerprint can provide a basis for quality evaluation of P. trichosanthis.

关 键 词：瓜蒌皮 指纹图谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]