荆芥-防风对LPS所致急性肺损伤小鼠模型的影响  

Effects of Compatibility of Schizonepetae Herba and Saposhnikoviae Radix on Lipopolysaccharide-induced Acute Lung Injury in Mice

作　　者：于柳[1] 王哲[1] 龚锡平[1] 杨靖[1] 武志强[1] 何敏[1] 史玉荣[1] 潘星[1] 王学[1] 曾南[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2014年第1期130-134,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973923);"2010年度高层次留学人才回国资助人选"项目;"2010年度留学回国人员科技活动项目择优资助"项目(国家人社部人社厅函[2010]411;412号)

摘　　要：目的:观察药对荆芥-防风的挥发油、含油水煎液、去油水煎液对内毒素(LPS)诱导的急性肺损伤(ALI)小鼠模型的影响,进一步揭示该药对的抗炎作用及物质基础。方法:采用小鼠ip内毒素(LPS,5 mg·kg-1)制备ALI模型。除地塞米松组(5 mg·kg-1)ip给药1次外,其余各组小鼠均连续ig给药5 d,每日1次,末次给药30 min后造模(空白组不进行造模)。注射LPS 6 h后,小鼠取血分离血清,并收集支气管肺泡灌洗液(BALF),剖取肺组织。测定小鼠全血白细胞分类计数,小鼠肺指数、肺湿/干重(W/D),BALF中总蛋白含量,肺组织髓过氧化物酶(MPO)活性、丙二醛(MDA)含量;血清白细胞介素6(IL-6)、白细胞介素10(IL-10)、肿瘤坏死因子α(TNF-α)水平。结果:药对荆芥-防风挥发油(45.19μL·kg-1)、含油水煎液(6.67g·kg-1,含油20μL)显著降低ALI小鼠全血白细胞(WBC)、淋巴细胞(LY)计数,降低肺指数、肺W/D,降低肺组织MPO活性与MDA含量及血清IL-10,TNF-α含量;去油水煎液(6.67 g·kg-1)明显降低全血WBC,LY计数,肺W/D及肺组织MDA含量。挥发油对血清IL-6水平亦显著降低。结论:药对荆芥-防风的挥发油、含油水煎液对ALI小鼠肺组织的炎性病变有较好的对抗作用,表现出抗炎效应,作用机制与抑制炎性细胞因子释放、抗氧化有关,其中药对挥发油部位可认为是其发挥抗炎效应的重要物质基础之一。Objective： To observe the effects of the volatile oil, decoction including oil and decoction without oil of the compatibility of Schizonepetae Herba and Saposhnikoviae Radix on lipopolysaccharide （LPS） - induced acute lung injury （ALI） in mice, and further reveal the anti-inflammatory effect and material basis of the compatibility. Method： The ALI model was established by intraperitoneal injection of LPS （5 mg .kg^-1 ） in mice. Except that dexamethasone group＇s mice were given the drugs once time by intraperitoneal injection before experiment, other groups＇ mice were given drugs by intragastric administration once a day for 5 days. Then, the model was established after 30 minutes of the last administration. Six hours after LPS injection, the blood, serum, bronchoalveolar lavage fluid （BALF） and lung tissue samples of mice were collected. White blood cell （WBC） count of blood, lung index, the ratio of wet lung to dry lung weight （ W/D）, myeloperoxidase （MPO） activity and malondialdehyde （MDA） content in lung tissue, total protein concentration in BALF, and the level of interleukin 6 （IL-6）, interleukin 10 （IL-10） and tumor necrosis factor-α （TNF-α） in serum were measured respectively. Result： The volatile oil （45.19 μL .kg^-1）, decoction including oil （6.67 g.kg^-1, including oil 20 μL） of the compatibility significantly decreased the white blood cell （WBC） count, lymphocyte （LY） count, as well as decreased the lung index, the value of W/D, MPO activity and MDA content in lung tissue and the level of IL-10 and TNF-α in serum. Decoction without oil （6.67 g .kg^-1） of the compatibility significantly decreased the WBC count, LY count and the value of W/D, MDA content in lung tissue. The volatile oil also decreased the level of IL-6 in serum. Conclusion： The volatile oil, decoction including oil of the compatibility of Herba Schizonepetae and Saposhntkoviae Radix show a protection action against the inflammatory pathological changes of lu

关 键 词：荆芥-防风 药对 内毒素 急性肺损伤 抗炎 

分 类 号：R285.5[医药卫生—中药学]