补肾养血方对卵巢早衰小鼠凋亡调控蛋白Bcl-2/Bax的影响  

Effects of Bushen Yangxue Prescription on Expression of Bcl-2 and Bax Protein in Mice Ovaries with Immune Premature Ovarian Failure

作　　者：董晓英[1] 柳顺玉[2] 李冬华[1] 周杰[1] 叶娜[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]首都医科大学附属北京妇产医院,北京100006

出　　处：《中国实验方剂学杂志》2014年第1期134-138,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81073096);首都医科大学基础临床合作课题(708016);北京中医药薪火传承"3+3"工程项目(2012-SZ-C-41)

摘　　要：目的:探讨补肾养血方对免疫性卵巢早衰(POF)小鼠卵巢凋亡调节基因B细胞淋巴瘤/白血病-2原癌基因(Bcl-2)、Bcl-2相关X蛋白(Bax)表达的影响。方法:以小鼠透明带3(Zp3)为抗原,皮下多点注射免疫BALB/c雌性小鼠,14 d后强化免疫,建立免疫性卵巢早衰模型。设补肾养血方低、中、高剂量(5.1,10.2,20.4 g·kg-1)进行治疗,以补佳乐(戊酸雌二醇0.6 mg·kg-1)为阳性对照,于造模后15 d开始灌胃给药1次/d,连续6周(每1周休息1 d)。放射免疫法检测小鼠血清激素水平,Western法检测卵巢组织Bcl-2,Bax蛋白的表达。结果:与空白对照组相比,模型组小鼠卵巢Bcl-2蛋白的表达减少,Bax蛋白表达增加,(P<0.05),模型组小鼠卵巢Bcl-2/Bax下降(P<0.01),提示模型组卵泡Bcl-2蛋白的低表达、Bax蛋白的高表达可能加速了卵泡的凋亡,导致卵巢早衰;与模型组相比,补肾养血方高、中、低剂量组及西药组小鼠卵巢Bcl-2蛋白的表达明显增加(P<0.01,P<0.05),卵巢Bax蛋白的表达均减少(P<0.05),Bcl-2/Bax明显降低(P<0.05)。结论:卵泡凋亡过快可能导致卵巢早衰,补肾养血方可能通过上调卵泡及卵巢间质Bcl-2蛋白的表达,下调Bax蛋白的表达,抑制卵泡的过快凋亡,从而改善卵巢功能。Objective： To study the effect of Bushen Yangxue prescription, on expression of B-cell lymphoma/leukemia-2 （Bcl-2） and Bcl-2 associated X protein （Bax） protein in ovaries of mice with immune premature ovarian failure （POF）. Method： BALB/c female mice model of POF was established by multiple sites subcutaneous injection of zona pellucida 3 of mice, and then the modeled mice were treated with low, middle and high dosage Bushen Yangxue prescription, and positive control medicine of Estradio Valerate. Follicle-stimulating （FSH）, 17-estradiol （E2）, lactogenic hormone （LH） were detected by radioimmunoassay method. Expression levels of Bcl-2 and Bax protein in mice ovarian were detected by western blot method. Result： Compared to Estradio Valerate group, FSH, E2, LH of low, middle and high dosage Bushen Yangxue prescription group were improved. Compared to the black control group, the expression of Bcl-2 protein of model group decreased obviously and the expression of Bax protein of model group increased obviously, Bcl-2/Bax ratio of model group decreased （P 〈 0.05）. Compared to the model group, the expression of Bcl-2 protein of high-dosage Bushen Yangxue prescription group and Estradio Valerate group increased obviously （P 〈 0.01 ） ; the expression of Bcl-2 protein of low and middle dosage Bushen Yangxue prescription group increased obviously （P 〈 0.05） , the expression of Bax protein of different dosage decreased obviously （P 〈 0.05）. All the data showed a dose-response relationship to some degree in expression of Bcl-2 protein within low, middle and high dosage Bushen Yangxue prescription group. Conclusion： It is indicated that rapid apoptosis of follicle may result in premature ovarian failure, and Bushen Yangxue prescription can improve the function of ovaries by up-regulating the expression of Bcl-2 protein and down-regulating the expression of Bax protein in ovarian.

关 键 词：补肾养血方 卵巢早衰 BCL-2 BAX 细胞蒯P 

分 类 号：R285.5[医药卫生—中药学]