盆炎平方内服联合蒲地方保留灌肠对慢性盆腔炎免疫状况及细胞因子的影响  

Effect of Penyanping Decoction Plus Pudi Decoction on Immune State and Cytokines of Chronic Pelvic Inflammation

作　　者：刘玉兰[1] 时菁静[1] 徐鸿雁[1] 吴松柏[1] 丁艳玲[1] 曲洪彬[1] 

机构地区：[1]承德医学院附属医院,河北承德067000

出　　处：《中国实验方剂学杂志》2014年第1期193-197,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省承德市科学技术研究与发展计划项目(20122183)

摘　　要：目的:探讨盆炎平方内服联合蒲地方保留灌肠对慢性盆腔炎(湿热瘀结型)患者的免疫调节作用。方法:将120例患者随机按数字表分为对照组和观察组各60例。对照组采用黄藤素分散片,4片/次,3次/d,自月经干净后开始服用,一个疗程20 d;苦参栓,1粒/次,1次/d,自月经干净后3 d开始用直肠给药,1个疗程连续使用10 d。观察组采用盆炎平方,1剂/d,常规水煎,每次约200 mL,从月经干净后分早晚服用,一个疗程20 d;蒲地灌肠方,1剂/d,自月经干净后3 d开始保留灌肠,1个疗程连续使用10 d。两组均连续观察3个月经周期。采用速率散射比浊法测定治疗前后血清免疫球蛋白G,A,M(IgG,IgA,IgM),采用单向免疫扩散法测定补体C3,C4水平,采用流式细胞仪法测定CD+3T细胞,CD+4细胞,CD+8T细胞及CD+4/CD+8;检测治疗前后白细胞介素2(IL-2)、白细胞介素10(IL-10)、肿瘤坏死因子-α(TNF-α)水平。结果:观察组IgG,IgA,IgM,C3及C4异常例数少于对照组(P<0.05);两组血清IgG,IgA,IgM水平下降,C3和C4水平升高(P<0.05或P<0.01);观察组IgG水平低于对照组,C3和C4高于对照组(P<0.05或P<0.01);观察组CD3,CD4,CD8,CD4/CD8异常例数均少于对照组(P<0.05);观察组CD3,CD4及CD4/CD8水平高于对照组,CD8低于对照组(P<0.05或P<0.0);治疗后两组血清IL-2,IL-10水平升高,观察组高于对照组,两组TNF-α下降,观察组低于对照组(P<0.01)。结论:盆炎平方内服联合蒲地方保留灌肠能调节慢性盆腔炎(湿热瘀结型)患者体液免疫,调节T细胞亚群,促进其平衡,还能调节炎性细胞因子水平,从而有利于疾病的康复,这可能是其重要的作用机制。Objective： To discuss the effect of Penyanping decoction plus Pudi decoction on the immunoregulation of patients with chronic pelvic inflammatory disease （hot and damp stasis junction）. Method： One hundred and twenty patients were randomly divided into observation group （n = 60） and control group （n = 60）. Control group was given Huangtengsu dispersible tablets after menstrual clean, 4 tablets each time, 3 time each day, for 20 days, matrine suppositories, 1 capsule each time, 1 time each day after menstrual clean for 3 days, for 20 days. Observation group was given Penyanping decoction after menstrual clean, 200 mL each dosage, 1 dosage each day, all for 20 days, Pudi decoction, 1 dosage each day after menstrual clean for 3 days, for 10 days. Both groups were observed for 3 menstrual cycle. Immune globulin G, immune globulin A, immune globulin M （IgG, IgA, IgM） of pretherapy and post-treatment were tested by rate nephelometry. Complement in blood serum （complement-C3, complement-C4） were measured by Single radil immunodiffusion. T lymphocyte subtype of CD3 , CD4＋, CD8 and CD4/CD8 were detected by flow cytometry. The level of interleukin-2, interleukin-10, tumor necrosis factor-a （IL-2, IL-10, TNF-a） were tested. Result： The cases of abnormal IgG, IgA, IgM, C3 and C4 in observation group were less than control group （P 〈 0.05） , the level of IgG, IgA, IgM of both groups after the treatment were lower than before, and the level of C3, C4 of both groups were higher than before （P 〈 0.05 or P 〈 0.01 ）. The level of IgG in observation group were lower than control group, and the level of C3 , C4 in observation group were higher ihan control group （P 〈 0.05 or P 〈 0：01 ）. The cases of abnormal CD3 , CD4＋ , CD8 , CD4/CDs＋ in observation group were less than control group （P 〈 0.05）, the level of CD3＋ , CD4＋ , CD4＋ / CD8＋ in observation group were higher than control group, the leVel of CDs＋ in observation group were lower than control group （

关 键 词：慢性盆腔炎 盆炎平方 蒲地方 免疫球蛋白 T细胞亚群 细胞炎性因子 湿热瘀结型 

分 类 号：R287[医药卫生—中药学]