甘蓝胚状体诱导成苗因素研究  

Study on Plant Regeneration from Microspore-derived Embryos in Cabbage(Brassica oleraceavar.capitata)

作　　者：高海娜[1] 王朝阳[1] 张恩慧[2] 

机构地区：[1]安康市农业科学研究所,陕西安康725000 [2]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《北方园艺》2014年第1期97-101,共5页Northern Horticulture

基　　金：西北农林科技大学重点推广资助项目(XTG-2009-17;XTG-2010-18);国家"十一五"科技支撑计划资助项目(2009BAD8B02)

摘　　要：以3个优良甘蓝品种为试材,进行了游离小孢子培养和胚状体植株再生技术研究,以期探讨不同甘蓝材料的基因型、胚状体类型、不定芽大小、烯效唑对不定芽诱导和生根的影响。结果表明:甘蓝子叶型胚容易发育单芽,鱼雷型胚和球型胚容易形成丛生芽;且单芽率和丛生芽率在不同基因型之间存在差异。高度大于2cm的不定芽转接到生根培养基上后生长速度先慢后快,生根数少且细长;小于2cm的不定芽生长速度先快后慢,生根数多且粗壮。小孢子不定芽在添加0.50mg/L烯效唑的MS+0.3mg/L NAA生根培养基中生根率最高且根系生长健壮,移栽成活率高达95.6%。Taking 3 superior cabbage varieties as test materials, isolated microspores culture and embryoid plant let regeneration technology were studied, the effects of different genotypes, different type of embryoids, different size of shoots, uniconazole on embryoids seedling induction were discussed. The results showed that cotyledon embryoids were easily form to single plants, and torpedo embryoids and ball-type embryoids were easily form to multiple shoots in all different genotypes. The rate of single plants and nmltiple shoots were different in different genotypes. The size of adventitious shoots was larger than 2 cm, the growth rate showed slow to fast after transferred to rooting medium,and the roots were fine and longer,The size of adventitious shoots shorter than 2 cm, the growth rate showed fast to slow after transferred to rooting medium,and the roots were healthy and strong. Add 0. 50 mg/L uniconazole MS＋0. 3 mg/L NAA regenerated plantlets rooting medium up to 95.6% survival rate of regenerated shoots thick stems and roots.

关 键 词：甘蓝 小孢子 子叶型胚 再生植株 

分 类 号：S635[农业科学—蔬菜学]