含氨基化碳纳米管的壳聚糖/β-甘油磷酸钠温敏凝胶的制备及性能研究  

Preparation and Properties of Chitosan/β-Glycerophosphate Thermosensitive Hydrogel Containing AminoCarbon Nanotubes

作　　者：郭亚可[1] 李晓娟[1] 熊庆[1] 孙丽[1] 陈文[1] 

机构地区：[1]新疆石河子大学药学院,新疆石河子832000

出　　处：《中国药学杂志》2014年第1期54-60,共7页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81160395)

摘　　要：目的制备含多壁碳纳米管/聚乙烯亚胺(MWCNTs-PEI)复合物的壳聚糖/β-甘油磷酸钠(CS/β-GP)温敏凝胶,为双缓释载体的研究提供一定基础。方法以壳聚糖温敏凝胶为载体,将多壁碳纳米管/聚乙烯亚胺分散到温敏凝胶中,制备含多壁碳纳米管/聚乙烯亚胺复合物的壳聚糖温敏凝胶,以胶凝时间为指标,考察β-甘油磷酸钠浓度、pH、温度和多壁碳纳米管/聚乙烯亚胺复合物质量对壳聚糖温敏凝胶的影响,采用扫描电镜(SEM)、红外光谱(FTIR)等对其表征,并初步考察其体内相容性。结果流变法测定其胶凝温度大约为37.0℃,在一定范围内,壳聚糖温敏凝胶胶凝时间随β-甘油磷酸钠浓度、pH、温度以及多壁碳纳米管/聚乙烯亚胺复合物质量的增加而缩短,且在动物体内均可形成凝胶,通过扫描电镜和红外光谱等实验发现,加入多壁碳纳米管/聚乙烯亚胺复合物后,其不与壳聚糖温敏凝胶发生化学反应,且壳聚糖温敏凝胶孔洞明显变小,结构致密,最终导致溶胀率和溶蚀速率的减小。结论含多壁碳纳米管/聚乙烯亚胺复合物的壳聚糖/β-甘油磷酸钠温敏凝胶具有快速凝胶化和良好的温敏性,可以作为良好的双缓释性载体。OBJECTIVE To synthesis chitosmn/β-glycerophosphate （CS/β-GP） thermosensitive hydrogel containing multi-walled carbon nanotubes-polyethyleneimine （MWCNTs-PEI） complexes and to lay a foundation for further research of dual slow-release deliv- ery system. METHODS Chitosan thermosensitive hydrogel containing MWCNTs-PEI was prepared by MWCNTs-PEI dispersed to the chitosan thermosensitive hydrogel. As the indicator of the gelling time, the experiment studied the effect of β-GP concentration, pH, temperature and MWCNTs-PEI composite quality on the theermosensitive chitosan hydrogel, and then it was charactered by using trans- mission electron microscopy （SEM）, infrared spectrometer （IR）, and initially investigated in vivo compatibility. RESULTS The dy- namic rheology method investigated the gelling temperature were about 37.0 ℃. Within a certain range, the gelling time of thermosen- sitive chitosan hydrogel was shortened with the increase of concentration of β-GP, pH, temperature, and the quality of MWCNTs-PEI complexes, and they could be transtormed into the hydrogel in vivo. The addition of MWCNTs-PEI complex didn＇t react chemically with the thermosensitive chitosan hydrogel and significantly make the holes of the chitosan thermosensitive hydrogel smaller by SEM and FT- IR, eventually leading to the swelling rate and the corrosional ratio decrease. CONCLUSION Chitosan/β-glycerophosphate thermo- sensitive hydrogel containing amino-carbon nanotubes has a rapid gelation and good temperature-sensitivity, which can serve as a good double sustained-release carrier.

关 键 词：温敏凝胶 碳纳米管 壳聚糖 β-甘油磷酸钠 聚乙烯亚胺 

分 类 号：R944[医药卫生—药剂学]