Orai1和STIM1在姜黄素诱导人肺腺癌A549细胞凋亡中的作用  

Effect of Orai1 and STIM1 on the apoptosis of human lung adenocarcinoma cells treated by curcumin

作　　者：林丹苗[1] 李文军[1] 徐蕾[1] 张洪梅[1] 邹飞[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院职业卫生与职业医学系,广东广州510515

出　　处：《现代预防医学》2014年第1期110-113,共4页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30971193)

摘　　要：目的探讨不同浓度姜黄素诱导人肺腺癌A549细胞凋亡中Orai1和STIM1mRNA水平的改变。方法 A549细胞分别暴露于5、10、15、20、25和30μmol/L姜黄素12、24和48 h后,应用四甲基偶氮唑蓝(MTT)比色法测定细胞增殖活力;将不同浓度姜黄素孵育A549细胞24 h后,流式细胞仪检测细胞凋亡率;real-time PCR确定25μmol/L姜黄素处理24 h后,Orai1和STIM1mRNA表达水平。结果姜黄素对A549细胞的抑制作用呈剂量依赖性和时间依赖性,不同浓度姜黄素处理A549细胞24 h细胞存活率分别为96.5%、95.0%、77.4%、63.9%、57%、39.6%,与对照组相比,差异有统计学意义(P<0.05或P<0.01)。姜黄素处理后,流式细胞仪分析结果显示,细胞凋亡率呈明显剂量依赖关系(P<0.05或P<0.01)。经姜黄素处理后A549细胞Orai1和STIM1mRNA表达量均明显低于对照组(P<0.01)。结论姜黄素可能引起A549细胞Orai1和STIM1 mRNA表达水平发生改变,通过下调Orai1和STIM1mRNA表达量引起细胞凋亡。Objective To study the effects of curcumin on the expressions of Orail and STIM1 mRNA in human lung adenocarcino- ma cells （A549）, and to discuss the molecular mechanism of pro-apoptosis of A549 induced by curcumin. Methods A549 cells were exposed to curcumin for 12, 24 and 48 hours at the concentrations of 5, 10, 15, 20, 25 and 30 μmol/L respectively. The cell viability was measured with MT＇F calorimetric assay. After the ceils were treated with curcumin, the apoptosis rates were detected by flow cytometry. The expressions of Orail and STIM1 mRNA were determined in A549 cells after exposure to 25 μmol/L for 24 hours by quantitative RT-PCR. Results The inhibitory effect of curcumin on A549 cells was shown in the dose-dependent and time-de- pendent manners. Compared with the control group （curcumin 0 μmol/L）, the survival rate of A549 cells were 96.5%, 95.0%, 77.4%, 63.9%, 57%, 39.6% （P〈0.05 or P〈0.01） respectively at different concentrations of curcumin after 24 hours exposure. Cell treated with curcumin and the apoptosis rates of A549 cells induced by curcumin were remarkably higher than those of the control cells. The mRNA expressions of Orail and STIM1 were remarkably decreased after being treated with curcumin （P〈O.01）. Con- clusion Curcumin may be able to change the expressions level of Orail and STIM1 mRNA in A549 cells, and through downgrading the mRNA expressions level of Orail and STIM1 to induce cell apoptosis.

关 键 词：Orai1 STIM1 人肺腺癌A549细胞 细胞凋亡 姜黄素 

分 类 号：R969.4[医药卫生—药理学]