At2g04450启动子的克隆和启动活性分析  

Molecular Cloning and Activity Analysis of a Promoter of an Arabidopsis thaliana Gene At2g04450

作　　者：吴坤鑫[1] 张秀春[1] 陈雄庭[1] 刘志昕[1] 彭明[1,2] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [2]农业部转基因植物及植物用微生物环境安全监督检验测试中心,海南海口571101

出　　处：《热带作物学报》2014年第1期53-58,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31070608;No.31201264)

摘　　要：为明确拟南芥At2g04450启动子对靶标基因表达的启动作用,根据已报道的拟南芥基因组序列设计合成1对引物,以拟南芥基因组DNA为模板克隆At2g04450上游调控序列,并与pBAR-GUS中间表达载体相连构建植物表达载体p04450p-GUS,采用农杆菌GV3101介导的渗透法转化野生型拟南芥,以GUS为报告基因研究该调控序列的组织表达特异性以及病原菌PstDC3000对该启动子的诱导表达效果。结果表明:获得At2g04450上游调控序列,该序列不仅具有启动子活性,并且具有组织特异性,GUS基因主要在根特异表达;病原菌PstDC3000对该启动子的表达没有诱导作用。本结果将为研究At2g04450的功能奠定基础。5＇-UTR of At2g04450 gene was cloned by PCR from Arabidopsis thaliana. To investigate the tissue expression pattern and the possible response to pathogen infection of the cloned regulatory sequence, an expression vector containing this sequence fused with GUS was constructed for transformation into A. thaliana by using agrobacterium-mediated method. Histochemical staining of transgenic A. thaliana showed that GUS reporter gene was predominantly expressed in roots, and the activity of the promoter can not be induced by the PstDC3000. This research will facilitate the study of At2g04450 gene function.

关 键 词：At2g04450启动子 GUS活性 组织表达特异性 

分 类 号：Q78[生物学—分子生物学]