荔枝古树胚性愈伤组织Fe-SOD成员基因克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of Fe-SOD Gene from Embryogenic Callus of an Ancient Litchi Tree

作　　者：练从龙[1] 赖钟雄[1] 卢秉国[1] 林玉玲[1] 冯新[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2014年第1期74-81,共8页Chinese Journal of Tropical Crops

基　　金：福建省农业科技平台(No.2008N2001);国家科技支持计划项目(No.2007BAD07B01)

摘　　要：为了解Fe-SOD在荔枝古树胚性愈伤组织保存中的分子机制,选择荔枝古树"宋荔"花药诱导而来的胚性愈伤组织为试验材料,采用同源克隆和RACE方法,获得荔枝Fe-SOD的两个转录本和基因组序列,并对该基因组的内含子进行分析。克隆获得长1 285 bp含有完整开放阅读框的荔枝Fe-SOD核酸序列,其编码1个含有250个氨基酸的蛋白质,将该序列命名为LcFe-SOD7a。生物信息学分析结果表明:该蛋白为稳定的、亲水的、含跨膜结构域的偏酸性蛋白质,定位于微体(过氧化物酶体)和细胞质,具有多样的磷酸化位点。LcFe-SOD7a基因组序列长2 284 bp,含7个内含子,其中第4个内含子较长,达920 bp。同时还得到其中的1条内含子驻留型可变剪接基因,该可变剪接基因提前终止,仅编码207个氨基酸的蛋白质,将该可变剪接基因命名为LcFe-SOD7b。Two different mRNA transcripts and their genomic sequence of Fe-Superoxide dismutase gene were obtained with homologous and RACE from embryogenic callus of an ancient litchi tree ＇Songli＇, and the bioinformatics were analyzed to illustrate its role in the process of in vitro reservation. The full length of LcFe- SOD eDNA was about 1 285 bp, containing a 753-nucleotides-long open reading frame （ORF） which encoding a protein of 250 amino acid residues, named as LcFe-SOD7a. Bioinformatics analysis showed that the protein encoded by LcFe-SOD7a with transmembrane helices was hydrophilie, stable and acidic protein, mainly located in the microbody （peroxisome） and cytoplasm, and had a variety of phosphorylation sites. The genomic sequence of LcFe-SODTa with a length of 2 284 bp was consisted of seven introns, with the fourth intron 920 bp, the longest. Meanwhile, an intron kept alternative splicing gene, named LcFe-SOD7b, which encoding a protein of 207 amino acid residues was obtained, which causing protein encoding terminated in advance.

关 键 词：荔枝古树 胚性愈伤组织 Fe—SOD 克隆 可变剪接 生物信息学分析 

分 类 号：S667.1[农业科学—果树学]