一种快速提取香蕉叶片总核酸、总RNA和总DNA的新方法  

A New Method of Total Nucleic Acid,Total RNA and Total DNA Isolation from Banana Leaves

作　　者：柴娟[1,2] 冯仁军[2] 史后蕊 任梦云 王静毅[2] 张银东[1,2] 

机构地区：[1]海南大学农学院,海南海口570228 [2]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101

出　　处：《热带作物学报》2014年第1期104-109,共6页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31201603);海南省自然科学基金项目(No.312050;No.312051);中央级公益性科研院所基本科研业务费项目(No.ITBB130201)

摘　　要：在SDS法的基础上,以富含多糖、多酚等次生代谢物质的香蕉叶片为材料,通过选择性添加适量5 mol/L KAc(pH4.8)或不同体积无水乙醇分别提取香蕉叶片总核酸、总RNA(不含DNA)和总DNA(不含RNA)。结果表明:不添加KAc的可提取到总核酸;添加1/3体积和2/3体积的KAc得到总RNA,添加1/3体积无水乙醇处理得到总DNA。该结果获得一种简单、快速,能在1次实验中添加不同试剂提取香蕉叶片总核酸、总RNA和总DNA的新方法。Total nucleic acid, total RNA （no DNA） and total DNA （no RNA） were isolated from banana leaves, with high polysaccharides, polyphenols and other secondary metabolites, using the method based on SDS by selectively adding suitable 5 mol/L KAc （pH4.8） and different volume of absolute alcohol. The results indicated that total nucleic could be isolated without KAc, and total RNA could be obtained with 1/3 or 2/3 volume of KAc, and total DNA could be gotten with 1/3 volume of absolute alcohol. Various nucleic acids extracted in one experiment could meet the demands of molecular biology experiment with adding the type of reagent. It is a fast and simple method.

关 键 词：香蕉叶片 总核酸 总RNA 总DNA 快速提取 

分 类 号：S668.1[农业科学—果树学]