和血通脉颗粒与功能主治有关的主要药效学实验研究  

Study on Main Pharmacodynamics Related to Functions of Hexue Tongmai Particles

作　　者：关乐[1] 杨关林[1] 张哲[1] 鲍文静[1] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2014年第1期47-50,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家科技重大专项(2010ZX09401-304);国家中医药行业科研专项(200807061)

摘　　要：目的:进行和血通脉颗粒主要药效学实验研究。方法:①大鼠舌静脉注射垂体后叶素(pit)造成急性心肌缺血,以J点升高1.5 mV,T波低平,双向,倒置,ST段下移0.5 mV,心律不齐为心肌缺血指标,计算各组动物心肌缺血百分率,观察药物对抗pit引起大鼠急性心肌缺血的作用;②两次皮下注射异丙肾上腺素(ISO)造成大鼠心肌缺血,以各时间点ST段偏移的总毫伏数为指标,观察药物对抗ISO引起大鼠心肌缺血的作用;③大鼠两次皮下注射盐酸肾上腺素注射液0.8 mL/kg,期间冰水刺激,造成大鼠血瘀模型,观察药物对大鼠血液流变学的影响;④通过检测大鼠血浆凝血酶时间(TT),凝血酶原时间(PT),活化部分凝血活酶时间(APTT),观察药物对大鼠血浆抗凝血作用;⑤通过检测大鼠血浆纤维蛋白原(Fib)含量,观察药物对大鼠血浆纤维蛋白溶解活性的作用。结果:①和血通脉颗粒明显降低垂体后叶素所致大鼠急性心肌缺血百分率;②明显减少异丙肾上腺素引起ST段偏移的总毫伏数(∑ST);③显著降低血瘀模型大鼠全血比黏度、血浆比粘度、红细胞压积及红细胞电泳时间;④明显延长大鼠血浆凝血酶时间、凝血酶原时间、活化部分凝血活酶时间,⑤明显降低大鼠血浆纤维蛋白原含量。结论:和血通脉颗粒大鼠低剂量组2.7 g(生药)/kg、中剂量组5.4 g(生药)/kg、高剂量组10.8 g(生药)/kg对心肌缺血有明显的保护作用;具有活血作用;具有抗凝血作用。Objective： To do the experimental study on the main pharmacodynamics of the Hexue Tongmai particles. Methods： Inject pituitrin into sublingual vein to establish the acute ischemie rats models. The raised up 1.5my J point, decreased T wave, biphasic T wave, inversed T wave elevated S - T Segment down 0.5mv and arrhythmias were applied as myocardial ischemia indices to the study. Calculate the percentage of myocardial ischemia of each group. Observe the effect of the drug on the cardiac ischemia rat induced by pit. Inject ISO twice subcutaneously to make the model of rats with acute myocardial ischemia. Take the total migration of ST segment of different time points as indexes. Observe the effect of the drug on the cardiac isehemia rat induced by ISO. Inject the adrenaline hydrochloride injection 0.8 mL/kg twice subcutaneously, stimulating the rats with ice water at the same time, in order to establish the rat model of blood stasis and to observe the effects of the drug on hemorheology of the rats. Detect TF,PT and AIrlT of the rats in plasma to ob- serve the drug＇ s anticoagulant function. Detect Fib content of the rats in plasma to observe the effects of the drug on the plasma fibrinolytic activity. Results ： Hexue Tongmai particles can reduce the myocardial ischemia percentage of the rat in- duced by pit. The drug can significantly reduce the total migration of ST segment induced by isoprenaline. The drug can significantly reduce the viscosity of whole blood, serum viscosity, packed cell volume and erythrocyte electrophoresis time of the rat model of blood stasis. The drug can significantly prolong the TF, PT and APT]？ of the rats in plasma. The drug can also significantly reduce the Fib content of the rats in plasma. Conclusion： The low dose group [ 2.7g（ crude drug）/ kg rats ], middle dose group [ 5.4g（ crude drug）/kg rats ], and high dose group [ 10.8g（ crude drug）/kg rats ] of Hexue Tongmai particles have obvious protective effect on myocardial isehemia, invigoration and coagulation.

关 键 词：和血通脉颗粒 药效学 胸痹心痛 痰瘀互结 Wistar大鼠 心肌缺血 活血 抗凝 

分 类 号：R285.5[医药卫生—中药学]