何首乌二苯乙烯苷含量差异性研究  

Research on Difference of 2,3,4,5'-tetra-hydroxy-stilbene-2-O-β-D-glucoside Content in Fallopia multiflora

作　　者：赵炜[1,2] 朱宽鹏[1,2] 生书晶[1,2] 姚焱[3] 赵树进[1] 

机构地区：[1]广州军区广州总医院药学部,广东广州510010 [2]华南理工大学生物科学与工程学院,广东广州510006 [3]广州大学生命科学学院,广东广州510006

出　　处：《中华中医药学刊》2014年第1期147-149,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：广东省自然科学基金项目(10151001002000012);广东省科技计划项目(00697862100303016)

摘　　要：目的：为筛选二苯乙烯苷含量差异显著的何首乌样本,为通过mRNA水平筛选与二苯乙烯苷合成相关酶基因,研究二苯乙烯苷合成途径打下基础。方法：HPLC,流动相为乙腈∶水（25∶75）;柱温：27℃;流速：1.0mL.min-1;进样量：10μL;紫外检测波长：320nm;进样时间：10min。结果：线性回归方程：y=41.233x＋1.6644,R2=1。结论：对水浴和室温冷浸两种萃取方法的比较发现水浴40min和冷浸12h效果无明显差异;冷浸12h和冷浸30h无显著差异;不同产地何首乌块根二苯乙烯苷含量差异显著（6.4%～0.0011%）,同一产地何首乌含量差异亦存在明显差异（6.4%～1.5%）;同株何首乌不同组织二苯乙烯苷含量存在较大差异,依次是：根〉茎〉叶;新叶〉老叶;老藤〉新藤。Objective：To screen samples the difference of 2,3,4,5＇ -tetra- hydroxy- stilbene- 2- O- β -D- glu- coside content for screening the genes correlated with the synthesis of 2,3,4,51 -tetra -hydroxy -stilbene -2 -O -β - D- glucoside at mRNA level and to research the pathway of 2,3,4,51 -tetrahydroxy- stilbene- 2 -O-β-D -glueoside synthesis. Methods.HPLC was used to determine the content. The mobile phase was H2O and CH3 CN with the ratio of 3 - 1 （v/v） over 10 rain. The flow rate was 1 mL/min, with detection at 320 nm. Results. The linear equation was ： y = 41. 233x ＋ 1. 6644, R2 = 1. Conclusion ：The comparison of water bath for 40 rain and quench at room temperature for 12 h shows that there is no obvious difference. There is no distinct difference between quench at room temperature for 12 h and 30 h either. The content is distinctly different in root tuber from different areas, and even different in root tubers from the same area. The content in different tissues is ： root 〉 stem 〉 leaf; young leaf 〉 old leaf; old stem 〉 young stem.

关 键 词：何首乌 二苯乙烯苷 含量差异 HPLC 

分 类 号：R284.1[医药卫生—中药学]