川芎嗪对蟾蜍离体坐骨神经干电生理特性的影响  

Effects of Ligustrazine on Electrophysiological Properties of Sciatic Nerve Stem of Toad

作　　者：王东吉[1] 尚改萍[1] 曹文君[2] 王黎敏[3] 李淑芬[1] 冯改壮[3] 

机构地区：[1]长治医学院生理教研室,山西长治046000 [2]长治医学院心血管病研究所,山西长治046000 [3]长治医学院机能实验室,山西长治046000

出　　处：《中华中医药学刊》2014年第1期170-172,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:应用电生理方法观察盐酸川芎嗪注射液对蟾蜍离体坐骨神经干电生理特性的影响。方法:将制备好的神经干置于标准任氏液中浸泡20min(作为对照组)后;随机再分3个实验组,即川芎嗪5mg、10mg和15mg浸泡组,各组又分别浸泡5、10、15、20、25、30 min 6个时间点。用BL-420E+生物机能实验系统测定各组各浸泡时间点神经干动作电位的阈强度、最适强度和不应期等电生理指标。结果:经不同浓度盐酸川芎嗪液处理后,引起神经干动作电位的阈强度和最适强度增大,不应期延长,与浸泡前(对照组)相比,差异极其显著(P<0.01)。3组浓度间比较:随川芎嗪液浓度增高对神经干电生理指标影响越明显(P<0.05,P<0.01)。经任氏液重新浸泡,消失的动作电位仍可恢复。结论:盐酸川芎嗪注射液能降低蟾蜍坐骨神经干的兴奋性,可逆性阻滞动作电位的产生。Objective ： The electrophysiological techniques were used to detect the effect of venom on electrophysiological properties of sciatic nerve stem of toad. Methods ： After the prepared toad＇s sciatic nerve was soaked in standard Ringer solution for 20 rain （as a control group） ,we divided it into three experimental groups, namely ligustrazine 5 mg, 10 mg and 15 mg group, and each group was soaked for 5,10,15,20,25,30 min respectively. The determination of electrophysiology indices of the different time points with threshold intensity, the biggest stimulus intensity and refractory period of action potential of the sciatic nerves from each group were measured respectively by BL - 420 E ＋ organism function experiment system. Results ： After treatment with different concentrations of ligustrazine hydrochloride solution, the threshold intensity of the nerve trunk action potential positive wave width, the optimum strength and refractory at each soak time points were increasing, and compared with unsoaked control group, the difference was extremely significant （P 〈 0.01 ）. The comparison among three different concentrations groups ： the higher voncentration of ligustrazine liquid, the greater impact on neural stem electrophysiological indicators （P 〈 0. 05,P 〈 0.01 ）. After soaked in Ringer＇ s solution, the action poten- tial which was disappeared can soil be restored. Conclusions： Ligustrazine hydrochloride injection can reduce the excitability of toad＇ s sciatic nerve stem and reversibly block the generation of the action potential.

关 键 词：盐酸川芎嗪 蟾蜍坐骨神经干 动作电位 阈强度 最适强度 不应期 

分 类 号：R285.5[医药卫生—中药学]