同步检测血浆和外周血单个核细胞中HCV RNA的临床研究  

Clinic Study of Synchronous Detection of the HCV RNA in Plasma and PBMC of Hepatitis C

作　　者：叶进[1] 刘薇[1] 刘劲松[1] 曾令兰[1] 

机构地区：[1]同济医科大学附属协和医院,武汉430022

出　　处：《中西医结合肝病杂志》2000年第5期5-6,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:探讨联合检测对提高HCV RNA在慢性丙型肝炎患者中检出率的临床意义。方法:利用逆转录聚合醇链反应(RT-PCR)技术对583例ALT升高持续1年以上的患者进行同步检测血浆和外用血单个核细胞(PBMC)中的HCV RNA。结果:血浆中HCV RNA总检出率为19.2%。PBMC中HCV RNA总检出率为24.5%.HCV RNA在PBMC中的检出率明显高于血浆中的检出率.结论:两种方法同时应用能够提高HCV RNA在慢性丙型肝炎患者中的检出率。Aim: To increase the HCV RNA detectable rate of the chronic Hepatitis C patients. Method: To detect synchronously the HCV RNA in Plasma and PBMC of Hepatitis C patients whose ALT keeping arising continually for more than one year by using the TR - PCR. Results the final detectable rate of plasma was 19. 2% and the final detectable rate of HCV RNA in PBMC was 24. 5% and the test result demonstrated that the detectable trai of HCV RNA in PBMC was higher than that of plasma. Conclusion; to detect synchronously HCV RNA in PBMC could increase the detectable rate of HCV RNA of Hepatitis C patients.

关 键 词：丙型肝炎病毒 血浆 丙型肝炎 RT-PCR PBMC 

分 类 号：R446.5[医药卫生—诊断学]