实验性癫癎大鼠脑MRI与病理学对照研究  

Comparative Study of MRI and Pathology of Epilepsy Caused Brain Injury in Rat

作　　者：叶静[1] 张文波[2] 祁吉[2] 韩悦[3] 廉宗澄[3] 

机构地区：[1]华西医科大学附属第一医院精神科,610041 [2]天津医科大学第一中心医院放射科 [3]天津医科大学第二医院MRI室

出　　处：《中国医学计算机成像杂志》2000年第5期344-346,共3页Chinese Computed Medical Imaging

摘　　要：目的：观察１．５Ｔ ＭＲＩ检出大鼠 性脑损伤的能力。材料和方法：Ｗｉｓｔａｒ大鼠３０只，毛果云香碱３５０ｍｇ／ｋｇ腹腔注射，诱发反复强直－痉挛全面大发作持续状态，ＨＥ染色，光镜下观察癫 持续不同时间脑组织损害程度，并与 １．５Ｔ ＭＲ成像对比（ＭＲＩ参数： ＳＥ序列 Ｔ１加权像：重复时间＝５００ｍｓ，回波时间＝ １５ｍｓ； ＦＳＥ序列 Ｔ２加权像：重复时间＝４００ｍｓ，回波时间＝１００ｍｓ；用表面线圈，层厚２．２ｍｍ，无间隔）。结果：癫 持续时间与脑损伤呈相关性改变。癫 持续 ３ｈ后，可见颞叶、海马区严重神经元脱失、胶质增生和脑水肿； ＭＲ扫描除在１只发作持续１８ｈ的大鼠左颞区检出长Ｔ１、长Ｔ２异常信号外（光镜提示为出血灶），未发现其余动物脑的结构异常和信号变化。结论：随着动物大发作持续时间延长， 性脑损害加重，大鼠癫 持续３ｈ以上，有明显的海马神经元坏死及胶质增生，形成典型的海马硬化；１．５Ｔ ＭＲ常规序列扫描尚不能检出癫 大鼠早期脑改变。Purpose: To observe the ability of demonstration of epilepsy caused brain injury with MRI in epilepto- genie rats. Materials and Methods: Thirty Wistar rats were injected with 10% pilocarpine intraperi- toneally to induce a generalized tonic - clonic seizure(GTCS). The degrees of brain injury with different duration of epilepsy were observed with light microscope and compared with 1. 5 T MRI findings(SE: T1 WI , FSE: T2 WI, Coil: surf are coil , Thickness: 2. 2mm without gap). Results: The duration of GTCS and the degree of brain injury in pathological study correlated fairly. After3 hours continuous GTCS, severe neuronal loss , ghosts and edema in the temporal lobe and hippocampal region were found by Pathology ,and no abnormality was revealed with MRI, MRI showed low signal intensity on T1 WI and high signal intensity on T2WI images in the left temporal lobe in one rat with GTCS lasting 18 hours. These abnormal signals were from hemorrhage confirmed by pathology.None of the remained rats was found abnormality on MRI. Conclusion: GTCS lasting for 3 hours can induce apparent neuronal less and ghosts, which means the formation mesial temporal sclerosis. The neuronal loss, gliosis and edema of brain in small animals such as rat cannot be demonstrated with conventional MRI sequences in acute epileptic animal models.

关 键 词：癫痫 磁共振成像 病理学 大鼠 

分 类 号：R445.2[医药卫生—影像医学与核医学]