甘肃马铃薯凝集素部分理化性质的研究  

Study on Some Physico-chemical Properties of the Lectin from Gansu Potato

作　　者：华绍烽[1] 李广林[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,730050

出　　处：《中国生化药物杂志》2000年第5期232-234,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：甘肃省自然科学基金资助项目!(ZR 96 0 47)

摘　　要：目的 :研究甘肃马铃薯凝集素的部分理化性质。方法 :采用SephadexG 15 0凝胶过滤法测定马铃薯凝集素 (STL)的分子量 ;加热法测定其热稳定性 ;硫酸 苯酚法测定中性糖含量 ;光谱法测定其紫外、红外光谱特征。结果 :该凝集素分子量为 10 2kD ,含有 48.5 %的中性糖 ,富含天冬氨酸。热稳定性试验表明 ,在 5 0℃以下对热不敏感 ,在 5 0℃以上对热敏感。光谱分析表明 :该凝集素的最大紫外吸收波长为 2 76nm ,在中红外区有三个特征吸收频率 16 40cm-1,3 15 0cm-1和 3 40 0cm-1。结论 :该研究为马铃薯凝集素的进一步研究、开发提供了科学依据。Purpose:The aim is to study some physico-chemical properties of Gansu potato lectin.Methods:The molecular weight of the lectin was estimated by gel filtration,and the carbohydrate content by the phenol-H 2SO 4 re agent.Heat stability was determined by heating,and the spectrum analysed by the UV and the IR.Results:The molecular weight of the lectin was estimated to be 102kD by g el filtration,the potato lectin was a glycoprotein which contained about 48.5% o f carbohydrate, and the lectin was insensitive to heat below 50℃ but sensitive over 50℃.The analysis of the spectrum showed that an absorption peak at 276 nm in th e UV spectra and the characteristic absorption peaks at about 1 640 cm -1 , 3 150 cm -1 , 3 400 cm -1 in the IR spectum respectively.Concl usion:The results provide a scientific basis for further study and utiliza tion of Gansu potato lectin.

关 键 词：马铃薯凝集素 理化性质 热稳定性 

分 类 号：R392－33[医药卫生—免疫学] Q513.202[医药卫生—基础医学]