灰枣和酸枣叶绿体trnL-trnF基因序列分析  

The Chloroplast Gene Sequence trnL-trnF of Ziziphus jujuba Mill. ‘huizao' and Ziziphus jujuba var. spinosa

作　　者：赵志浩[1] 王杰[2] 李振山[2] 管少华[1] 李继东[1] 

机构地区：[1]河南农业大学林学院,郑州450002 [2]商丘学院风景园林学院,河南商丘476113

出　　处：《河南科学》2013年第11期1883-1886,共4页Henan Science

基　　金：国家林业公益性行业科研专项(200904024);郑州市科技领军人才项目(10LJRC179)

摘　　要：以灰枣和酸枣叶片为材料,用改良的CTAB法提取总DNA,用通用引物对枣叶绿体trnL基因内含子和trnL-trnF间隔区进行PCR扩增,得到了trnL基因内含子和trnL-trnF间隔序列片段,并对trnL-trnF间隔序列片段回收测序,测序结果符合植物叶绿体基因trnL-trnF间隔序列特征,用DNAMAN软件进行同源性分析,结果表明灰枣和酸枣trnL-trnF基因序列同源性为94.78%.枣和酸枣的分类学地位是一个长期争议的问题,初步开展了灰枣和酸枣叶绿体基因序列分析的研究,为研究枣和酸枣分子系统进化提供参考.In this article we collect the leaf of Ziziphus jujuba Mill.‘huizao’ and Ziziphus jujuba var. spinosa （Rhamnaceae）,to isolate the total DNA with modified CTAB method. The trnL gene intron and trnL-trnF intergenic spacer are amplified with general primers. We sequence the trnL-trnF intergenic spacer and the results satisfy the character of plant cpDNA. The phytoplasma analysis with DNAMAN software shows 94.78%homology between the two samples. Since the argument on the classification of jujube and sour jujube,the study could provide some reference for future research.

关 键 词：灰枣 酸枣 叶绿体基因 序列分析 trnL-trnF间隔序列 

分 类 号：S665.1[农业科学—果树学]