IGF-1通过ERK-MNK信号通路诱导骨髓间充质干细胞表达VEGF的实验研究  

Experiment study of IGF-1 induces VEGF expression by ERK-MNK signaling pathway in bone marrow mesenchymal stem cells

作　　者：刘柏含 高艳霞[2] 宫培刚 杨小琳[2] 王占义[3] 

机构地区：[1]长春市口腔医院三马路门诊部,吉林长春130051 [2]吉林大学基础医学院 [3]长春市口腔医院,颌面外科

出　　处：《中国实验诊断学》2013年第12期2123-2125,共3页Chinese Journal of Laboratory Diagnosis

基　　金：本文由吉林省卫生厅项目基金支持,项目编号：2009z083

摘　　要：目的 为探讨ERK-MNK信号通路是否参与胰岛素样生长因子-1（IGF-1）诱导骨髓间充质干细胞（BMSCs）表达VEGF,进一步揭示MSCs与VEGF的作用机制.方法 通过免疫印迹方法检测经IGF-1诱导的骨髓间充质干细胞内HIF-1α、p-MNK等信号蛋白及VEGF的表达变化,给予MNK抑制剂CGP 57380和HIF-1α抑制剂YC-1后再检测含外源性IGF-1的BMSCs中VEGF的表达水平.结果 外源性IGF诱导后,HIF-1α等信号蛋白表达上调,分别阻断MNK和HIF后,VEGF的表达降低.结论 IGF-1通过调节ERK-MNK信号通路从而诱导BMSCs表达VEGF.Objective IGF-1 is one of the most abundant growth factors in bone marrow stromal cells,which , as well as VEGF,plays an important role in promoting new bone formation or bonehealing process. To investigate＇how to induce VEGF, exogenous IGF-1 was added to bone marrow stromal cells in vitro,then some signaling protein lnhibitors was used to determine the role of signaling proteins related. Methods By western blot,the expression of VEGF and signaling proteins-HIF-la, p-MEK,p-ERK,p-MNK and p-elF-4E was detected. The experiment was divided into four groups： （1） control group, （2） 50 ng/ml IGF-1, （3） CGP 57380- MNK inhabitor,and （4） 50 ng/ml IGF-1 and 5ng/ml HIF-lainhabitor YC-1. Results Expression of these sinaling proteins and VEGF were upgraded after exogenous IGF- 1,while increasing expression of VEGF is blocked by MNK inhabitor or HIF-lainhabitor . Conclusion IGF-1 regula- ted the expression of VEGF through ERK-MNK signal transduction.

关 键 词：胰岛素样生长因子-1 血管内皮生长因子 ERK-MNK 骨髓间充质干细胞 骨组织工程 

分 类 号：Q813.11[生物学—生物工程]