脂蛋白相关磷脂酶A_2酶活性水平及其I198T基因多态性与2型糖尿病的相关性研究  

Association between activity and I198T of lipoprotein-associated phospholipase A2 and Type 2 Diabetes Mellitus

作　　者：岳志红[1] 贾玫[1] 李珊珊[1] 

机构地区：[1]北京大学人民医院检验科,北京100044

出　　处：《中国实验诊断学》2013年第12期2178-2182,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的 探讨脂蛋白相关磷脂酶A2酶活性水平及I198T多态性是否是2型糖尿病的危险因素.方法应用TaqMan-ARMS PCR方法,检测117例2型糖尿病患者（T2DM组）和203例健康对照组I198T基因多态性,并对Lp-PLA2酶活性水平、各种生化指标以及临床基本资料进行测定和调查.结果 2型糖尿病组Lp-PLA2酶活性水平明显高于对照组,差异有统计学意义（29.44（16.10,55.81）,21.39（12.42,52.82）,19.77（14.63,26.61） nmol·ml-1·min-1,P〈0.01）;校正各项混杂因素（CHO,TG,Hs-CRP和 Lp（a））后,Lp-PLA2酶活性的最高组与最低组相比,2型糖尿病的OR值为12.21（95%CI,2.88~51.80）;Lp-PLA2酶活性水平在基因型II的Lp-PLA2酶活性最高[22.79（15.49,36.11） nmol·ml-1·min-1,P〈0.05],基因型TT的Lp-PLA2酶活性最低[15.70（8.83,22.60） nmol·ml-1·min-1,P〈0.05）,基因型IT与2型糖尿病的关联显著（OR：2.13 CI：（1.11-4.07） P〈0.05）.结论 Lp-PLA2的酶活性水平在2型糖尿病患者中显著升高,是其独立的危险因子; 基因型IT与2型糖尿病的关联显著.Objective To investigate whether the I198T and activity of Lp-PLA2 were the risk factors of Type 2 di- abetes mellitus. Methods A case-control study, According to the amplification refractory mutation system （ARMS- PCR）combined with a TaqMan fluorogenic probe, to detect the I198T gene polymorphism, respectively, to test and in- vestigate 117 people with Type 2 diabetes mellitus and 203 controls of Lp-PLA2 activity, a various biochemical index and clinical basic information. Results Type 2 diabetes mellitus had significantly higher Lp-PLA2 activity than the con- trois（29.44（16.10,55.81）,21.39（12.42,52.82）,19.77（14.63,26.61） nmol ml-1 min-1 ,P〈0.01,respectively） after additional adjustment for various traditional risk factors, including ages, sex, CHO, TG, Hs-CRP, and Lp （a）, the highest quartile of Lp-PLA2 activity comparison to the bottom quartile, the OR was 12.21 （95 % CI, 2.88 -- 51.80） for Type 2 .diabetes mellitus; Lp-PLA2 activity ir genotype II was highest （22.79（15.49,36.11） nmol m1-1 min-1 ,P d0.05） and in genotype TT was the lowest （15.70（8.83,22.60） nmol ml-1 . min -1 ,P〈0.05） ,the association be- tween genotype IT and coronary artery disease was significant（P〈0.05）. Conclusion Lp-PLA2 activity was signifi- cantly higher in Type 2 diabetes mellitus group and was a risk factor for Type 2 diabetes mellitus; the association be- tween genotype IT and Type 2 diabetes mellitus was significant.

关 键 词：单核苷酸多态性 2型糖尿病 脂蛋白相关磷脂酶A2 

分 类 号：R587.1[医药卫生—内分泌]