系统性红斑狼疮患者血清miR-181a检测的临床意义  

Serum miR-181a detection in systemic lupus erythematosus patients and its clinical significance

作　　者：陈明坤[1] 石磊[1] 谷明莉[1] 朱俊[1] 郭方宇[1] 汪怀周[1] 秦琴[1] 邓安梅[1] 

机构地区：[1]第二军医大学长海医院实验诊断科,上海200433

出　　处：《中国实验诊断学》2013年第12期2201-2204,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家重点基础研究发展计划(973)资助项目(2013CB531606);国家自然科学基金资助项目(81273282;81202353;81371786;30972730);上海科委基金资助项目(11JC1410902);吴阶平基金(320.6750.13147);上海杨浦区人才发展资金(鼎元资金);长海医院基金资助项目(CH125530300);南京军区医药卫生科研基金(12MA056)

摘　　要：目的 研究系统性红斑狼疮（SLE）患者血清miR-181a检测的临床意义.方法 采用反转录PCR法检测了30例SLE患者和30健康个体血清miR-181a,分析了其与患者血沉、C反应蛋白、抗dsDNA抗体和SLEDAI-2K评分等临床特征的关系.结果 SLE患者和健康个体血清miR-181a的相对表达量分别为177（P25~P75：78~354）和41（P25~P75：23~91）,差异具有统计学意义（Z=4.26,P〈0.01）.SLE患者血清miR-181a与血沉、C反应蛋白、抗dsDNA抗体和SLEDAI-2K评分呈正相关,相关系数分别为0.52、0.51、0.63和0.55（P值均小于0.01）.结论 血清miR-181a是评估SLE病情的有益标志物.Objective To investigate the clinical value of serum miR-181a detection in systemic lupus erythematosus （SLE） patients. Methods Serum miR-181a in 30 SLE patients and 30 healthy individuals were detected by RT-PCR. The association between serum raiR-181a and clinical characteristics of. SLE, including erythrocyte sedimentation rate （ESR） ,C-reactive protein （CRP） ,anti-dsDNA antibody and SLEDAI scores,were analyzed. Results The median rela- tive expression of serum miR-181a in SLE patients were 177 （P25 -P75：78- 354）, which was higher than that in healthy controls （median： 41 ; P25 - P75 ： 23 - 91）. Serum miR-181a was positively correlated with ESR, CRP, anti-dsD- NA antibody and SLEDAI-2K cores in SLE patients, with the correlation coefficient of 0. 52,0. 51,0. 63 and 0.55 （P〈 0.01 for all）. Conclusion Serum miR-181a was a useful marker for SLE disease activity estimation.

关 键 词：系统性红斑狼疮 微小RNA 疾病活动度评估 

分 类 号：R593.241[医药卫生—内科学]