山西白猪SLA-DQB基因多态性研究  

Polymorphism of SLA-DQB Gene in Shanxi White Pigs

作　　者：杨文平[1] 张家琦[1] 曹果清[2] 

机构地区：[1]河北联合大学生命科学学院,中国河北唐山063000 [2]山西农业大学动物科技学院,中国山西太谷030801

出　　处：《生命科学研究》2013年第6期525-527,547,共4页Life Science Research

基　　金：山西省科技攻关项目(991016;20080311031);国家科技部农业科技成果转化资金资助项目(2008GB2A300032)

摘　　要：为评价和选育山西白猪提供分子基础数据,采用PCR(polymerase chain reaction)-RFLP(restriction fragment length polymorphism)法对202头山西白猪SLA(swine leukocyte antigen)-DQB基因外显子2的273 bp扩增产物多态性进行研究.山西白猪经HaeⅢ酶切后产生6种基因型:167 bp/23 bp/29 bp/54 bp(AA)、84 bp/83bp/23 bp/29 bp/54 bp(BB)、167 bp/4 bp/102 bp(CC)、167 bp/23 bp/83 bp(EE)、167 bp/23 bp/29 bp/54 bp/4 bp/102 bp(AC)和167 bp/106 bp/4 bp/102 bp(CD);5种等位基因(A、B、C、D和E),其中等位基因A和基因型AA有明显优势.山西白猪SLA-DQB基因外显子2 HaeⅢ-RFLP带型较多.Polymorphism of the exon 2 of SLA （swine leukocyte antigen）-DQB gene in 202 Shanxi white pigs was analyzed by PCR （polymerase chain reaction）-RFLP （restriction fragment length polymorphism） method to provide molecule data for the evaluation and breeding of Shanxi white pigs. A uniform fragment of 273 bp was obtained in Shanxi white pigs. The 273 bp PCR product was digested by Hae Ⅲ and genetic polymorphism was investigated with PCR-RFLP. Six kinds of genotypes were found in Shanxi white pigs, 167 bp/23 bp/29 bp/54 bp （AA）, 84 bp/83 bp/23 bp/29 bp/54 bp （BB）, 167 bp/4 bp/102 bp （CC）, 167 bp/23 bp/83 bp （EE）, 167 bp/23 bp/29 bp/54 bp/4 bp/102 bp （A C） and 167 bp/106 bp/4 bp/102 bp （CD）. The exon 2 of SLA-DQB gene digested by Hae Ⅲ could be divided into five kinds of alleles （A, B, C, D and E）. Allele A and genotype AA in Shanxi white pigs were superior genes. There were rich band patterns of the exon 2 of SLA-DQB gene digested with Hae Ⅲ in Shanxi white pigs.

关 键 词：山西白猪 SLA-DQB基因 PCR-RFLP 

分 类 号：S828[农业科学—畜牧学] Q75[农业科学—畜牧兽医]