塞来昔布减轻糖尿病大鼠心肌细胞纤维化的实验研究  

Experimental study of celecoxib alleviating diabetic myocardial fibrosis in rats

作　　者：李若男[1] 温俊平[1] 林桂芳[1] 吕琦[1] 杨小玲[1] 陈宁[1] 

机构地区：[1]福建医科大学教学医院福建省老年医院内分泌科,福州350003

出　　处：《福建医药杂志》2013年第6期54-56,共3页Fujian Medical Journal

基　　金：福建省卫生厅青年科研基金资助项目(2009-2-56)

摘　　要：目的探讨塞来昔布对糖尿病心肌病大鼠心肌细胞纤维化的影响。方法将Wistar大鼠30只随机分为对照组(A组)、糖尿病心肌病组(B组)和糖尿病心肌病塞来昔布治疗组(C组)各10只。取大鼠心肌组织,经常规石蜡切片、染色后测定胶原纤维面的密度值;采用RT-PCR法测定TGF-β1mRNA表达情况。结果与A组相比,B组大鼠心肌组织的胶原纤维密度值、TGF-β1mRNA表达明显升高(P<0.05)。C组大鼠的胶原纤维、TGF-β1mRNA表达明显低于A组和B组(均P<0.05)。结论塞来昔布可能通过抑制TGF-β1减轻糖尿病大鼠心肌纤维化。Objective To investigate the effect of celecoxib on myocardial fibrosis in diabetic rats. Methods Wistar rats were randomly divided into normal control group （A group, n= 10）, diabetic myocardial group （B group, n= 10） and diabetic cardiomyopathy celecoxib treatment group （C group, n= 10）. The rats were sacrificed and myocardial tissues underwent paraffin and staining tissue sections, the surface density of collagen fibers level was detected. The expression of transforming growth factor-βl （TGF-β1） mRNA was detected by RT-PCR. Results Compared with the normal control group, the collagen fibers of myocardial tissue in diabetic rats, TGF-β1 mRNA expression was significantly increased （P〈0. 05）. The collagen fibers in celecoxib treated rats, TGF-β1 mRNA expression was significantly lower than the diabetic myocardial group and normal control group （P〈0. 05）. Conclusion Celecoxib can alleviate development of myocardial fibrosis in diabetic rats.

关 键 词：塞来昔布 糖尿病心肌病 胶原纤维 转化生长因子-Β1 

分 类 号：R587.2[医药卫生—内分泌]