不同溶液中制备的L-半胱氨酸自组膜的红外反射吸收光谱研究  

Infrared reflection-absorption spectroscopy investigation on the self assembled monolayers of L-cysteine on a gold substrate

作　　者：冯春梁[1] 邱文媛 孙越[1] 张诗慧 

机构地区：[1]辽宁师范大学化学化工学院功能材料化学研究所,辽宁大连116029

出　　处：《辽宁师范大学学报（自然科学版）》2013年第4期507-511,共5页Journal of Liaoning Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(60572009);辽宁省教育厅高校重点实验室项目(2008S134)

摘　　要：在金表面上制备L-半胱氨酸(L-Cys)自组装单分子膜(SAMs)已引起人们的广泛兴趣.但是,我们发现,不同自组装溶液对SAMs的形成具有很大影响.利用偏振模式傅立叶变换红外反射吸收光谱对分别在磷酸盐缓冲溶液(PBS)、醋酸盐缓冲溶液(ABS)以及盐酸与氢氧化钠混合溶液中制备的L-Cys SAMs的结构进行了研究.结果表明,在不同溶液中制备L-Cys SAMs的最佳酸度条件不同,在ABS中为pH 5.0,在盐酸与氢氧化钠混合溶液中为pH 6.0,而在PBS中L-Cys SAMs的表面密度随着pH的增加而增大.就所研究的3种溶液而言,在优化条件下,在ABS中制备的L-Cys SAMs的表面密度最大,因而其表面结构最完美.本研究为进一步制备免疫传感器、酶传感器等生物传感器提供了有利的技术支持.L-Cysteine （Cys） is one of most the interesting thiolated amino acid candidates for self-as-sembled monofilm （SAMs） formation on the gold electrode .However ,we have found that the forma-tion of Cys SAMs would be effected by the type of aqueous media .In this paper ,the structures of Cys SAM s prepared on gold substrate in phosphate buffered solution （PBS ） ,acetate buffer solution （ABS） ,solution of sodium chloride and sodium hydroxide （NaOH/HCl） have been studied using Po-larization-modulation Fourier transform infrared reflection-absorption spectroscopy （PM-FT-IRRAS） respectively .The results showed that the optimum cyclic condition for preparation of Cys SAMs on the gold surface prepared in ABS was pH5 .0 ,in NaOH/HCl was pH6 .0 ,and in PBS the Cys SAM s density increases with the increase of pH .The Cys SAMs prepared in ABS has a maximum surface density in the three solutions ,so the structure of the Cys SAMs is most perfect .Therefore ,ABS is an ideal self assembling solution .T he results provide technical support for the preparation of chemical sensors ,immunosensors and enzyme sensors .

关 键 词：L-半胱氨酸 自组装单分子膜 生物传感器 

分 类 号：TS251.51[轻工技术与工程—农产品加工及贮藏工程]