单甲氧基聚乙二醇化学修饰人血清白蛋白及产物性能表征  

Characterization of Human Serum Albumin Modified by Monomethoxy(Polyethylene Glycol)

作　　者：杨庆华[1] 冯乙巳[2] 徐超[2] 

机构地区：[1]合肥工业大学医学工程学院,安徽合肥230009 [2]合肥工业大学化学工程学院,安徽合肥230009

出　　处：《安徽化工》2013年第6期22-25,共4页Anhui Chemical Industry

摘　　要：用三光气活化的单甲氧基聚乙二醇5000(mPEG)和N-羟基琥珀酰亚胺(NHS)反应制备单甲氧基聚乙二醇琥珀酸亚胺酯(SC-mPEG),SC-mPEG修饰人血清白蛋白(HSA)制备mPEG-HSA。傅里叶变换红外光谱仪(FT-IR)、紫外分光光度计(UV)和高效液相色谱仪(HPLC)对产物结构予以分析表征。结果表明,已经成功得到目标产物,mPEG的平均活化度为75%,SC-mPEG与HSA的平均偶联度为45%。SC-mPEG is synthesized by N-hydroxysuccinimide and monomethoxy（polyethylene glycol）5000 activated with his （trichloromethyl）carbonate which is used to couple with human serum albumin （HSA）. The structure of the product is determined by FT-IR , UV and HPLC. Results show that the desired product is successfully synthesized. The average activation ratio of mPEG is about 75% , and the average modification ratio of SC-mPEG is about 40%.

关 键 词：人血清蛋白 单甲氧基聚乙二醇 化学修饰 表征 

分 类 号：R331[医药卫生—人体生理学]