东方田鼠感染日本血吸虫前后血清IL-4水平的检测  

Detection of the Levels of IL-4 in Sera from Bred Microtus Fortis before and after Infection by Schistosoma Japonicum

作　　者：张新跃[1] 何永康[1] 李毅[1] 罗新松[1] 喻鑫玲[1] 

机构地区：[1]湖南省寄生虫病防治研究所,岳阳414000

出　　处：《实用预防医学》2000年第5期332-333,共2页Practical Preventive Medicine

基　　金：卫生部血吸虫病重点项目(总理基金 )!(94-Y- 02);科技部"九.五"攻关!(1996-A2 3-06-04)资助

摘　　要：[目的 ]　检测东方田鼠感染日本血吸虫前后血清白细胞介素 - 4(IL- 4)的水平。 [方法 ]　EL ISA法比较检测室内繁殖东方田鼠和昆明系小鼠未感染 (BMF0 、AM0 )与感染日本血吸虫第 9天 (BMFi9、AMi9)、16天 (BMFi1 6 、AMi1 6 )血清 IL- 4的水平。 [结果 ]　BMFi9血清 IL- 4水平 (131.94pg/ ml)显著高于 BMF0 (5 2 .5 9pg/ ml)和 BMFi1 6(5 8.16 pg/ ml) ;与昆明系小鼠相比较 ,BMF0 是 AM0 (12 .43pg/ ml)的 4.2倍 ,BMFi9是 AMi9(4 4.5 3pg/ ml)的 3.0倍。[结论 ]　 BMF血清 IL - 4水平显著高于 AM,且于感染早期显著升高 ,表明 IL -Objective] To detect the levels interleukin-4(IL-4) in sera from laboratory bred Microtus forits(BMF) before and after infection by Schistosoma japonicum.[Methods] Indirect ELISA was performed to detect the levels of IL-4 in sera from BMF and albino mice (AM) before infection by shistosoma japonicum (BMF 0, AM 0) and 9 d(BMF i9 ,AM i9 ) and 16 d(BMF i16 ,AM i16 ) after infection.[Result] The average level of IL-4 in sera of BMF i9 (131.94 pg/ml) was significantly higher than those of BMF 0 (52.59 pg/ml) and BMF i16 (58.16 pg/ml), and compared with AM, that of BMF 0 was 4.2 times that of AM 0 (12.43 pg/ml) and that of BMF i9 3.0 times that of AM i9 (44.53 pg/ml).[Conclusion] The average level of IL-4 of BMF was significantly higher than that of AM, and during the early stage of infection, the levels of IL-4 of BMF increased significantly, possibly showing that IL-4 played an important role in the resistance of M. fortis to S. japonicum infections.

关 键 词：东方田鼠 日本血吸虫 IL-4 检测 

分 类 号：R383.24[医药卫生—医学寄生虫学]