极端耐盐植物盐穗木脱水胁迫响应基因Rd22的克隆及表达分析  

Cloning and Expression Analysis of Dehydration-respective Protein Gene Rd22( HcRd22) from Extremely Salt-tolerant Plant Halostachys caspica

作　　者：张霞[1] 常丹[1] 彭丹[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《生物技术》2013年第6期5-8,共4页Biotechnology

基　　金：国家973计划前期研究专项("区域特有或濒危生物资源的保护与利用基础";编号:2012CB722204);新疆生物资源基因工程重点实验室开放课题("盐穗木耐盐相关基因的功能鉴定";编号:XJDX0201-2012-05)资助

摘　　要：目的:选择合适的标记基因用于检测胁迫处理盐穗木的有效性。方法:根据NCBI同源序列比对设计简并引物,通过RT-PCR从盐穗木(Halostachys caspica)中克隆获得盐穗木脱水胁迫响应基因Rd22(HcRd22)基因,并采用实时定量PCR技术检测其逆境胁迫下的表达规律。结果:最终扩增获得301bp盐穗木HcRd22核酸序列,NCBI和clustalwz序列比对分析表明HcRd22具有BURP结构域。400 mmol/L盐胁迫和50μmol/L ABA处理分别使HcRd22的基因表达量在处理12h上调2.5倍和3倍。结论:所克隆的HcRd22基因属于盐胁迫和ABA处理的响应基因,可作为今后研究盐穗木胁迫处理的标准基因。Objective：In order to choose an proper marker gene to assess the effeicieny of abiotie stress treatment on the extremely euhalo- phyte Halostachys caspica. Method：A dehydration - respective protein Rd22 was obtained from H. caspica （HeRd22） by homology cloning strategy. And real -time quantitative PCR were used to determine the gene expression pattern under abiotic stress. Result：The obtained HeRd22 eDNA contained a 301 bp nueleotides ,and was predicted to encode a putative peptide with a conserved BURP motif through NCBI and clustalwz analysis. The HeRd＇22 transcripts accumulated to 2. 5 and 3.0 fold with 400 mmol/L NaC1 and 50 μmol/L ABA treatment at 12 h respectively. Conclusion ：HeRd22 were identified as a ABA -and NaCI stress -responsive gene, and could examine the efficiency of abiotic stress treatment on H. caspica as a standard marker gene.

关 键 词：盐穗木 脱水胁迫响应蛋白基因Rd22 同源克隆 实时定量PCR 

分 类 号：Q781[生物学—分子生物学]