布鲁氏菌外膜蛋白基因omp19序列克隆与原核表达  

Sequence Cloning and Prokaryotic Expression of omp19 Outer Membrane Protein Gene in Brucella

作　　者：杜志强[1] 林涛[1] 王建英[1] 

机构地区：[1]内蒙古科技大学数理与生物工程学院生物工程与技术研究所,内蒙古包头014010

出　　处：《生物技术》2013年第6期8-10,共3页Biotechnology

摘　　要：目的:研究猪型布鲁氏菌外膜蛋白基因omp19的序列性质,同时,进行目的基因的原核表达。方法:以猪型布鲁氏菌基因组作为模板进行基因序列的扩增,利用大肠杆菌原核表达系统进行重组表达。结果:猪型布鲁氏菌外膜蛋白基因omp19的开放阅读框序列全长为534 bp,演绎177个氨基酸。诱导表达的重组蛋白质的理论分子量为17.6 kD,而且实际大小与理论值一致。结论:成功表达了Omp19分子,为进一步研究Omp19分子的免疫保护作用奠定了基础。Objective： The sequence properties of swine Brucella ompl9 gene were studied,and this gene was expressed at the same time, using E. coli prokaryotie expression system. Method： Swine Brucella genome was taken as the template for gene sequence amplification, and ompl9 gene was recombinantly expressed using E. coli prokaryotic expression system. Result： The ORF sequence of ompl9 gene in- cluded 534 bp, and it could encoded 177 amino acids. The molecular weight of this recombinant molecule Ompl9 was 17. 6 kD, and it was consistent with the theoretical value. Conclusion： The Ompl9 molecule was successfully expressed, and these work laid foundation for thefurther study on immune protective function of Ompl9.

关 键 词：猪型布鲁氏菌 外膜蛋白质 omp19基因 序列克隆 原核表达 

分 类 号：Q786[生物学—分子生物学]