转cry1Ab/vip3H+epsps基因粳稻对非靶标害虫稻叶蝉田间种群的影响  

Impacts of transgenic cry1Ab/vip3H+epsps japonica rice on the population dynamics of its non-target herbivores,rice leafhoppers,under field conditions

作　　者：卢增斌[1] 韩乃顺 徐刚[1] 刘玉娥[1] 胡萃[1] 彭于发[2] 郭予元[2] 叶恭银[1] 

机构地区：[1]浙江大学昆虫科学研究所/水稻生物学国家重点实验室/农业部作物病虫分子生物学重点开放实验室,杭州310058 [2]中国农业科学院植物保护研究所植物病虫生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2013年第11期1275-1285,共11页Acta Entomologica Sinica

基　　金：国家转基因生物培育重大科技专项(2011/2013ZX08011-001)

摘　　要：转基因作物可能带来的风险之一是对非靶标生物尤其是非靶标植食者产生潜在的影响。本研究采用吸虫器取样法,通过在浙江长兴2地点3年的试验评价了新型抗虫/耐除草剂转cry1Ab/vip3H+epsps基因粳稻(G6H1)及其亲本对照(秀水110,XS110)对稻叶蝉田间种群动态的影响。结果表明:叶蝉类主要有黑尾叶蝉Nephotettix cincticeps(Uhler)、二点叶蝉Cicadula fascifrons(Stl)和电光叶蝉Deltocephalus dorsalis Motschulsky组成,其中黑尾叶蝉是优势种。虽然3种叶蝉的种群密度随着地点和年份的不同有所不同,但是转基因水稻对3种叶蝉种群密度的年度变化均没有显著性影响。少数年份,黑尾叶蝉成虫、若虫及其两者总密度的时间动态在转基因水稻和对照田之间存在差异,大多数年份,趋势一致且无显著差异(P>0.05)。另外,二点叶蝉和电光叶蝉种群的时间动态在转基因水稻和对照田之间也相似。综合评价认为,本供试转基因水稻品系G6H1对稻田稻叶蝉种群无明显的负面影响。One of the risks posed by transgenic crops is their potential effects on non-target orgamsms, especially non-target herbivores. In this study, experiments were conducted at two sites in Changxing, Zhejiang, eastern China in three years to assess the effects of transgenic crylAb/vip3H ＋ epsps japonica rice （G6H1） and its non-transgenic rice （Xiushui 110, XSll0） on rice leafhoppers using the vacuum- suction machine method under field conditions. The results showed that rice leafhopper species included Nephotettix cincticeps （Uhler）, Cicadula fascifrons （Still） and Deltocephaltts dorsalis Motschulsky, of which N. cincticeps was the dominant species. Population densities of these three leafhopper species varied depending on tested years and tested sites. However, the tested transgenic rice line did not significantly influence the population densities of these leafhopper species. Population dynamics of N. cincticeps adults and nymphs as well as their total density were similar between transgenic and non- transgenic rice plots in most tested years, although some significant differences were observed in a few tested years. In addition, population dynamics of C. fascifrons and R. dorsalis were also not significantly affected by transgenic rice. In general, the tested transgenic rice line G6H1 has no markedly negative impact on the population of these three rice leafhopper species in the field.

关 键 词：转基因水稻 CRYLAB vip3H基因 epsps基因 稻叶蝉 黑尾叶蝉 二点叶蝉 电光叶蝉 种群动态 

分 类 号：Q968[生物学—昆虫学]