AOPP对ECV304细胞表达SDF-1α的影响及其可能的机制  

Effects of advanced oxidation protein products on expressions of stromal cell derived factor-1α in ECV304 cells and the possible mechanism

作　　者：魏明丽[1] 丁怀玉[2] 吕田[1] 李真[1] 于红玖[1] 王梅[1] 马裴裴[1] 

机构地区：[1]大连医科大学附属第一医院干部综合科,辽宁大连116011 [2]大连医科大学附属第一医院心内科,辽宁大连116011

出　　处：《大连医科大学学报》2013年第6期543-546,共4页Journal of Dalian Medical University

摘　　要：目的观察晚期氧化蛋白产物(advanced oxidation protein product,AOPP)对内皮细胞表达基质细胞衍生因子-1α(stromal cell derived factor-1α,SDF-1α)的影响,并探讨其作用机制。方法 ECV304细胞分别经0,50,100,200μmol/L AOPP处理后,用RT-PCR法检测其SDF-1αmRNA的表达,观察AOPP对ECV304细胞的SDF-1α表达的影响。ECV304细胞先经NF-κB通路特异性阻断剂BAY11-7082处理,再经AOPP处理,用RTPCR法检测其SDF-1αmRNA的表达,观察BAY11-7082对AOPP促ECV304细胞SDF-1αmRNA表达作用的影响。结果 AOPP促进ECV304细胞SDF-1αmRNA表达,并且随AOPP浓度的升高,SDF-1αmRNA的表达增加。对照组、AOPP 50μmol/L组、AOPP 100μmol/L组及AOPP 200μmol/L组SDF-1αmRNA/GAPDH mRNA值分别为0.034±0.005、0.109±0.019、0.226±0.037及0.322±0.043,组间比较差异均有显著性意义,P<0.05。BAY11-7082抑制AOPP促ECV304细胞SDF-1αmRNA表达,两组细胞SDF-1αmRNA/GAPDH mRNA值分别为0.256±0.035和0.322±0.043,P<0.05。结论 AOPP可上调ECV304细胞表达SDF-1α,NF-κB通路可能是介导这一作用的重要途径之一。Objective To observe the effect of advanced oxidation protein product （ AOPP） on the expression of SDF-1αin ECV304 cells,and to investigate the possible mechanism .Methods SDF-1αmRNA expressions were revealed by RT -PCR respectively in ECV304 cells preincubated with different concentrations of AOPP .SDF-1αmRNA expression was re-vealed by RT-PCR in ECV304 cells preincubated with BAY 11-7082 which was a inhibitor of NF -κB signal transduc-tion pathway .Results SDF-1αexpression was constitutionally detected in ECV 304 cells and was up-regulated by AOPP in a concentration-dependent manner .The promoting effect of AOPP on the expression of SDF -1αin ECV304 cells could be inhibited by BAY11-7082.Conclusion AOPP could up-regulate SDF-1αexpression in ECV304 cells by NF-κB signal transduction pathway .

关 键 词：晚期氧化蛋白产物 基质细胞衍生因子-1Α NF-κB 

分 类 号：R54[医药卫生—心血管疾病]