当归多糖对银屑病患者外周血单一核细胞NF-κB及IFN-γ的影响  

The influence of angelica polysaccharide on the expression of NF-κB and IFN-γ in co-culture of peripheral blood mononuclear cells and keratinocytes from psoriatic patients

作　　者：景海霞[1] 周辉[1] 段德鉴[1] 唐吉云[1] 喻标[1] 

机构地区：[1]湖北医药学院附属太和医院皮肤科,十堰442000

出　　处：《中国麻风皮肤病杂志》2013年第12期769-772,共4页China Journal of Leprosy and Skin Diseases

基　　金：湖北医药学院研究生启动基金项目(编号:2007QDJ26)

摘　　要：目的:确定体外当归多糖对银屑病患者外周血单一核细胞(PBMC)与角质形成细胞(KC)NF-κB表达及IFN-γ分泌的影响。方法:将正常人KC+银屑病患者PBMC混合培养(A组),正常人KC和正常人PBMC混合培养作为作对照(B组)。当归多糖作用于两组,采用Western Blot检测混合细胞中NF-κB的蛋白表达水平。双抗体夹心ELISA法检测培养上清液中IFN-γ的水平。结果:A组混合培养体系中NF-κB p65蛋白表达水平及上清液中IFN-γ水平明显高于B组(P<0.01);经当归多糖作用后,A组混合培养体系中NF-κB p65蛋白表达水平及上清液中IFN-γ水平较处理前降低(P<0.05),而B培养体系中NF-κB p65蛋白表达水平及上清液中IFN-γ水平较处理前升高(P<0.05)。结论:当归多糖可能通过抑制NF-κB活化、减少IFN-γ分泌,对银屑病治疗发挥作用。Objective： To determine the influence of angelica polysaccharide on the expression of NF-KB and IFN-γin a co-culture of peripheral blood mononuclear ceils （PBMC） keratinocytes （KC） from psoriatic patients. Methods： After PBMC and KC were cocultured, angelica polysaccharide was added into the culture medium, and then, proteins were extracted from the mixed ceils to detect NF-KB p65 expression by western blot and supernatants were collected to determine the IFN-γ levels by double-antibody sandwich ELISA. Re- suits： The levels of NF-KB p65 protein and IFN-γ in mixed culture of KC and psoriatic PBMC were signifi- cantly higher than those in KC and normal PBMC （P〈0.01）. The levels of NF-KB p65 protein and IFN-γ in KC and psoriatic PBMC were significantly decreased after the treatment of angelica polysaccharide compared with untreated group （P〈0.01） ; however the levels of NF-KB p65 protein and IFN-γin KC and normal PB- MC were increased than untreated group （P〈0.05）. Conclusion： Angelica polysaccharide may have a thera- peutic effect on psoriasis by inhibiting NF-KB activation abrogated IFN-γ secretion.

关 键 词：当归多糖 外周血单一核细胞 角质形成细胞 核转录因子-ΚB 干扰素-Γ 

分 类 号：R275[医药卫生—中医皮科]