转草莓GalUR基因提高拟南芥和生菜维生素C含量  

Enhancement of vitamin C contents in Arabidopsis thaliana and lettuce by expressing GalUR gene from strawberry

作　　者：陶启长[1] 郭新波[1] 孙小芬[2] 唐克轩[1,2] 

机构地区：[1]复旦大学生命科学学院,上海200433 [2]上海交通大学农业与生物学院,上海200240

出　　处：《上海农业学报》2013年第6期14-19,共6页Acta Agriculturae Shanghai

基　　金：国家863高新技术项目(2011AA100605)资助

摘　　要：在模式植物拟南芥和经济作物生菜中过量表达维生素C(AsA)合成的旁路途径——古洛糖途径的关键酶D半乳糖醛酸还原酶基因(GalUR),探讨该基因对植物AsA含量的贡献和作用。结果表明:同非转基因对照相比,转草莓GalUR基因的拟南芥中AsA含量最高提高到2.59倍,转草莓GalUR基因的生菜中AsA含量最高提高到2.11倍。The effect of D-galacturonic acid reductase gene（GalUR,a key gene of synthesizing vitamin C in gulose pathway） on improving AsA content of plants was investigated by overexpressing GalUR in Arabidopsis thaliana and lettuce.The results showed that compared with non-transgenic plants,the transgenosis of GalUR from strawberry to A.thaliana and lettuce plants increased both plants＇ AsA contents by up to 1.59 times and 1.11 times respectively.

关 键 词：拟南芥 生菜 维生素C 代谢工程 

分 类 号：Q78[生物学—分子生物学]