机构地区:[1]无锡市第三人民医院消化内科,江苏省无锡市214041 [2]中国人民解放军第二军医大学附属长海医院消化内科,上海市200433
出 处:《世界华人消化杂志》2013年第35期3947-3953,共7页World Chinese Journal of Digestology
基 金:江苏省"333工程"科研基金资助项目;No.CAE01101-06;江苏省中医药局中医药科技基金资助项目;No.LZ11127;无锡市社会发展基金资助项目;No.CSZ00N1110~~
摘 要:目的:探讨大黄治疗大鼠重症急性胰腺炎(severe acute pancreatitis,SAP)肠动力衰竭的可能机制.方法:将54只♂SD大鼠随机分为假手术组(SO组)、重症急性胰腺炎肠动力衰竭组(SAP组)和大黄治疗组,每组18只.经胆胰管内加压注射3.5%牛磺胆酸钠0.1 mL/100 g体质量制备SAP肠动力衰竭模型,治疗组在造模前给予10%的大黄汤剂(2 mL/100 g体质量)灌胃1次,SAP组和SO组则灌注等量0.9%氯化钠溶液.每组于造模后3、6、12 h各处死6只大鼠并立即心脏取血,检测血清淀粉酶、脂肪酶水平,酶联免疫吸附试验检测血清5-羟色胺(5-hydroxytryptamine,5-HT)含量.同时检测6h时间点各组大鼠距屈氏韧带10 cm空肠、末端回肠及乙状结肠组织中5-HT1R、5-HT2R的表达情况.结果:造模后SAP组(淀粉酶分别为9807 U/L±698 U/L、15442 U/L±952 U/L、18231 U/L±869 U/L,脂肪酶分别为94 U/L±23 U/L、257 U/L±99 U/L、306 U/L±107 U/L,5-HT分别为1192 ng/mL±142 ng/mL、1437 ng/mL±114 ng/mL、1646 ng/mL±163 ng/mL)和治疗组(淀粉酶分别为2238 U/L±298 U/L、2313U/L±302 U/L、2289 U/L±323 U/L,脂肪酶分别为62 U/L±16 U/L、71 U/L±17 U/L、69 U/L±15 U/L,5-HT分别为794 ng/mL±86ng/mL、814 ng/mL±83 ng/mL、798 ng/mL±81 ng/mL)血清淀粉酶、脂肪酶及5-HT水平均明显高于SO组(淀粉酶分别为1853 U/L±272 U/L、1959 U/L±269 U/L、1897 U/L±293 U/L,脂肪酶分别为46 U/L±9 U/L、54U/L±11 U/L、52 U/L±14 U/L,5-HT分别为699 ng/mL±64 ng/mL、721 ng/mL±76 ng/mL、705 ng/mL±72 ng/mL)(P<0.01),且每个时间点治疗组均较SAP组显著降低(P<0.01).与SO组相比,SAP组5-HT1、5-HT2R蛋白及阳性细胞面积(SAP组空肠、末端回肠及乙状结肠5-HT1R+细胞面积分别为4.58万m2±0.56万m2、4.64万m2±0.63万m2、4.79万m2±0.56万m2,5-H T2R+细胞面积分别为4.89万m2±0.61万m2、4.76万m2±0.51万m2、5.03万m2±0.73万m2;SO组空肠、末端回肠及乙状结肠5-HT1R+细胞面积分别为5.63万m2±0.82万m2、5.94万m2±0.91万m2、6.37�AIM: To investigate the effect of Da-Huang on intestinal motility failure in rats with severe acute pancreatitis (SAP) and the potential mech-anism involved. METHODS: Fifty-four SD rats were randomly divided into s sham operation (SO) group, a SAP group and a Da-Huang treatment group, with 18 rats in each group. Gastrointestinal mo-tility disturbance in rats with SAP was induced by retrograde injection of sodium taurocholate (0.1 mL/100 g) into the bitiopancreatic duct. Before modeling, the rats in the treatment group received 10% Da-Huang solution (2 mL/100 g), and the rats in other two groups received 0.9% NaCI solution. The rats were sacrificed at 3, 6 and 12 hours (6 rats at each time), and blood samples were taken for detecting the levels of amylase, lipase and the contents of 5-hydroxy-tryptamine (5-HT) by enzyme-linked immu- nosorbent assay. The jejunum, terminal ileum and sigmoid colon tissues 10 cm apart from the trans-ligament were collected at 6 h for examin-ing the expressions of 5-HT1 receptor (5-HT1R) and 5-HT2 receptor (5-HT2R). RESULTS: The levels of serum amylase, lipase and 5-HT at three time points in the SAP group (amylase: 9807 IU/L ± 698 IU/L, 15442 IU/L ± 952 IU/L, 18231 IU/L ± 869 IU/L; lipase: 94 IU/L ± 23 IU/L, 257 IU/L ± 99 IU/L, 306 IU/L ± 107 IU/L; 5-HT: 1192 ng/mL ± 142 ng/mL, 1437 ng/mL ± 114 ng/mL, 1646 ng/mL ± 163 ng/mL) and treatment group (amylase: 2238 IU/L ± 298 IU/L, 2313 IU/L ± 302 IU/L, 2289 IU/L ± 323 IU/L; lipase: 62 IU/L ± 16 IU/L, 71 IU/L ± 17 IU/L, 69 IU/L ± 15 IU/L; 5-HT: 794 ng/mL ± 86 ng/mL, 814 ng/mL ± 83 ng/mL, 798 ng/mL ± 81 ng/mL) were significantly higher than those in the SO group (amylase: 1853 IU/L ± 272 IU/L, 1959 IU/L ± 269 IU/L, 1897 IU/L ± 293 IU/L; li-pase: 46 IU/L ± 9 IU/L, 54 IU/L ± 11, 52 IU/L ± 14 IU/L; 5-HT: 699 ng/mL ± 64 ng/mL, 721 ng/ mL ± 76 ng/mL, 705 ng/mL ± 72 ng/mL)(all P 〈 0.01), but they were lower in the treatment group than in the SAP gr
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