落新妇甙对缺血再灌注损伤肝脏HO-1表达的影响  

Effect of astilbin on HO-1 expression in hepatic warm ischemia-reperfusion injury

作　　者：慕宁[1] 江艺[1] 吕立志[1] 

机构地区：[1]中国人民解放军南京军区福州总院肝胆外科,福建省福州市350025

出　　处：《世界华人消化杂志》2013年第35期4019-4023,共5页World Chinese Journal of Digestology

基　　金：福建省自然科学基金重点资助项目;No.2011Y0046~~

摘　　要：目的:通过观察落新妇甙对肝脏热缺血再灌注损伤中血红素加氧酶1(heme oxygenase-1,HO-1)表达的影响,探讨其缺血再灌注保护作用的分子机制.方法:C57BL/6小鼠随机分为4组(n=8):假手术组(Sham)、模型组(I/R)、落新妇甙小剂量(10 mg/kg)干预组和落新妇甙大剂量(40 mg/kg)干预组.缺血前24和1 h干预组小鼠腹腔注射分别给予10或40 mg/kg的落新妇甙,建立肝左、中叶70%部分肝缺血再灌注模型,模型组和假手术组给予同样体积的生理盐水.小鼠肝脏左叶缺血90 min、再灌注6 h后各实验组采集血液和肝脏组织样本.检测血清中丙氨酸转氨酶(alanine transaminase,ALT)活性,Western blot检测肝组织中核因子B(nuclear factor B,NF-B)和HO-1蛋白含量,半定量RT-PCR检测上述分子mRNA表达情况.结果:落新妇甙小、大剂量干预后血清ALT含量较I/R对照组均明显下降(假手术组:142U/L±25 U/L;模型组:3521 U/L±270 U/L;落新妇甙小剂量干预组:1766 U/L±179 U/L;落新妇甙大剂量干预组:1067 U/L±101 U/L,P<0.01);落新妇甙小、大剂量干预组肝组织中NF-B蛋白表达与I/R模型对照组比较均渐次降低;落新妇甙小、大剂量干预组肝组织中HO-1蛋白表达与I/R模型对照组比较均渐次升高,亦与半定量RT-PCR结果相符(假手术组:0.53 U/L±0.07 U/L;模型组:1.00 U/L±0.11 U/L;落新妇甙小剂量干预组:1.17 U/L±0.16 U/L;落新妇甙大剂量干预组:1.57 U/L±0.07 U/L小剂量干预组P<0.05;大剂量干预组P<0.01).结论:落新妇甙干预能降低缺血再灌注损伤(ischemia/reperfusion injury,IRI)引起的血清A LT水平升高;落新妇甙干预能降低I R I肝组织中NF-B蛋白的高表达,促进IRI肝组织HO-1蛋白和mRNA的表达,显示其保护作用与促进HO-1的表达密切相关.AIM： To study the molecular mechanism be-hind the effects of astilbin in protecting the liver against warm ischemia-reperfusion injury. METHODS： C57BL/6 mice were randomly di-vided into four groups （n = 8 for each）： a sham-operated group （Sham）, a model control group （I/R）, a low dose astilbin treatment group （10 mg/kg） and a high dose astilbin （40 mg/kg） treatment group. At 24 h and 1 h before isch-emia, mice in the two treatment groups were intraperitoneally injected with 10 and 40 mg/kg astilbin, respectively. Then 70% hepatic isch-emia-reperfusion, including the left and middle hepatic lobes, were induced. The I/R model con-trol group and the sham-operated group were administered with equal volume of normal sa-line. After 90 min ischemia and 6 h reperfusion of the partial hepatic lobe, blood and liver tissue samples were collected. Serum ALT activity was detected, and the levels of NF-κB and HO-1 in liver tissues were detected by Western blot. The mRNA expression of these molecules was de-tected by semiquantitative RT-PCR. RESULTS： Compared with the I/R model con-trol group, serum ALT in both low and high dose treatment groups significantly decreased （sham： 142 U/L ± 25 U/L; I/R： 3521 U/L ± 270 U/L; low dose group： 1766 ± 179U/L; high dose group： 1067 U/L ± 101 U/L, P 〈 0.01）, and the decline was more significant in the high dose group than in the low dose group （P 〈 0.01）. The protein content of NF-~cB in liver tissues gradual- ly decreased in both treatment groups compared with the I/R model control group, and was low- er in the high dose group than in the low dose group. HO-1 levels gradually increased in treat- ment groups, and the increase was more signifi- cant in the high dose group than in the low dose group. The results of mRNA expression showed a similar trend （sham： 0.53 ± 0.07; I/R： 1.00 ± 0.11; low dose group： 1.17 ± 0.16; high dose group： 1.57 ± 0.07, P 〈 0.05 or 〈 0.01）. CONCLUSION： Intervention with astilbin can reduce

关 键 词：丙氨酸转氨酶 核因子-B血红素加氧酶1 Western BLOT 半定量RT—PCR 

分 类 号：R285.5[医药卫生—中药学]