策勒黑羊BMPR-IB、MTNR1A和PTGS2基因的RFLP分析  

RFLP Analysis of BMPR- IB,MTNR1A and PTGS2Gene in Cele Black Sheep

作　　者：王琼[1] 邵勇钢[1] 刘玲玲[1] 田佳[1] 古丽尼沙.吐拉甫 努孜古丽.图尔荪 耿明[1] 莫合塔尔.夏甫开提 刘武军[1] 

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2013年第7期1330-1339,共10页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高技术研究发展项目"新疆绵羊品种主要经济性状功能基因的鉴定"(201111115)

摘　　要：【目的】寻找与绵羊多胎性状相关的DNA标记,为提高绵羊繁殖力的标记辅助选择提供科学依据。【方法】试验以策勒黑羊为主要研究对象,利用PCR-RFLP、DNA测序等技术,对BMPR-IB、MTNR1A和PTGS2基因的遗传多态性进行研究。【结果】策勒黑羊BMPR-IB基因具有多态性,存在三种基因型:++、B+和BB;MTNR1A和PTGS2基因不存在多态。【结论】验证了BMPR-IB基因的A746G突变位点是策勒黑羊多胎性的主效基因,而MTNR1A和PTGS2基因与策勒黑羊繁殖性能关系不大。[Objective] Looking for DNA mark about sheep prolificacy trait,it is to provide the theoretical basis for the development of sheep fertility marker assisted selection （MAS）.[Method] In this experiment,the polymorphism of BMPR-IB,MTNR1A and PTGS2 gene in Cele black sheep was detected by PCR-RFLP and DNA sequencing.[Result]The results showed,that BMPR-IB gene in Cele black sheep had polymorphism,with three kinds of genotype：＋ ＋,B ＋ and BB; MTNR1A and PTGS2 gene didnt have polymorphism.[Conclusion]The fact that mutational site of A746G of BMPR-IB gene is prolificacy major gene in Cele black sheep is checked,not MTNR1A and PTGS2 gene.

关 键 词：策勒黑羊 多胎性状 BMPR-IB MTNR1A PTGS2 

分 类 号：S826[农业科学—畜牧学] S188.2[农业科学—畜牧兽医]