利用体外产气法研究苜蓿青贮与玉米青贮组合效应的瘤胃发酵特征  

Rumen Rermentation Characteristics of Vitro Gas Production of Alfalfa Silage and Corn Silage Combination Effect

作　　者：史卉玲[1] 王连群[1,2] 席琳乔[1,2] 刘慧[1] 刘美华[1] 景春梅[1] 马春晖[3] 

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]石河子大学动物科技学院,新疆石河子832000

出　　处：《新疆农业科学》2013年第7期1353-1359,共7页Xinjiang Agricultural Sciences

基　　金：现代农业产业技术体系建设专项资金(CARS35);"十二五"科技支撑计划项目(2010BAD05B01)

摘　　要：【目的】在研究苜蓿青贮和玉米青贮组合效应的瘤胃发酵特征。【方法】苜蓿青贮与玉米青贮分别以0∶100(AS0)、20∶80(AS20)、40∶60(AS40)、60∶40(AS60)、80∶20(AS80)、100∶0(AS100)六个比例组合,通过体外产气法培养72 h,研究不同组合效应对瘤胃培养液发酵的pH值、氨态氮浓度(NH3-N)、降解率、产气量和挥发性脂肪酸(VFA)产量的影响。【结果】72 h发酵结束时,各试验组体外降解液pH值均在7.17～7.58,AS20显著低于AS0,极显著低于其它试验组(P<0.01);AS80的NH3-H值极显著高于AS0、AS20(P<0.01),显著高于AS40、AS60和AS100(P<0.05);体外降解率AS80最高,AS20、AS40、AS60、AS100之间差异不显著(P>0.05);体外产气量随苜蓿青贮的比例增加而增加,其中AS100和AS80较高;AS60、AS80的乙酸、丙酸、丁酸和总挥发性脂肪酸均极显著高于AS0(P<0.01);乙酸/丙酸和乙酸/总挥发性脂肪酸中,AS20最低(P<0.01),其它各组间差异不显著(P>0.05)。【结论】苜蓿青贮和玉米青贮不同比例组合可提高粗饲料的利用效率,其中AS80组比例最佳。[Objective] The purpose of this experiment is to study the rumen fermentation characteristics of the combined effects of alfalfa silage and corn silage.[Method]Alfalfa silage and corn silage were 0∶100（AS0）,20∶80 （AS20）,40∶60 （AS40）,60∶40 （AS60）,80∶20 （AS80）,100∶0 （AS100） six proportional combination,through in vitro gas production method for 72 h culture,the effects of different combinations of rumen pH value of liquid fermentation,the concentration of ammonia nitrogen （NH3-N）,the degradation rate,gas production and volatile fatty acids （VFA） production were researched.[Result]The results showed that the end of the 72 h fermentation,each test group in vitro degradation of pH values were between 7.17 to 7.58,AS20 was significantly lower than AS0 and extremely lower than the other test groups （P 〈 0.01） ; the AS80 NH3-H value was extremely higher than AS0,AS20 （P 〈 0.01）,significantly higher than the AS40,AS60 and AS100 （P 〈 0.05） ; between the in vitro degradation rate AS80 was the highest,and the difference between AS20,AS40,AS60 and AS100 was not significant （P 〉 0.05） ; vitro gas production increased with the increase in the proportion of alfalfa silage,which AS100 and AS80 were higher; AS60,AS80 acetic acid,propionic acid,butyric acid and total volatile fatty acids were significantly higher than AS0 （P 〈 0.01）;Among the acetic acid / propionic acid and acetic acid / total volatile fatty acids,AS20 was the lowest （P 〈0.01）,and the difference between the other groups was not significant （P 〉 0.05）.[Conclusion] It is concluded that the different proportions of alfalfa silage and corn silage combination can improve the efficiency of utilization of roughage,among which AS80 group proportion was the best.

关 键 词：苜蓿青贮 玉米青贮 瘤胃发酵 

分 类 号：S816.11[农业科学—饲料科学]