RNA干扰假基因Cripto-3表达对大肠癌细胞增殖和侵袭的影响  

RNA interference in pseudogene Cripto-3 on proliferation and invasion of human colon cancer cell

作　　者：朱灵[1] 蒋鹏程[1] 满昌峰[1] 彭辉勇[1] 徐娟[1] 范钰[1] 

机构地区：[1]江苏大学附属人民医院肿瘤研究所,江苏镇江212002

出　　处：《江苏大学学报（医学版）》2013年第4期284-287,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81250035);江苏省"333工程"基金资助项目(BRA2012103);江苏省创新基金资助项目(CX-LX12-0679);镇江市社会发展基金资助项目(SH2011031)

摘　　要：目的:探讨RNA干扰下调假基因Cripto-3(Cr-3)对人大肠癌细胞SW480增殖和侵袭的影响。方法:将大肠癌细胞随机分为5组:空白对照组,空载对照组,siRNA转染组(5 nmol/L组、10 nmol/L和20 nmol/L组)。将Cr-3小干扰RNA(small interfering RNA,siRNA)转染人大肠癌SW480细胞后,采用实时荧光定量PCR检测Cr-3表达水平;分别采用平板克隆形成实验和Transwell法检测大肠癌细胞的增殖和侵袭能力。结果:与空白对照组和空载对照组比较,siRNA转染组Cr-3表达水平均明显下降,且呈浓度依赖性(P<0.05),癌细胞克隆形成和穿过滤膜的细胞均明显减少,且与浓度相关(P<0.05)。结论:假基因Cr-3在大肠癌细胞增殖侵袭中起着重要的作用,可能是大肠癌诊疗中的一个重要靶点。Objective： To study the effects of RNA interference-mediated silencing of pseudogene Cripto3（Cr-3） on proliferation and invasion of colon cancer cells.Methods： The cells were divided into five groups,blank control group（Con-A group）,empty control group（Con-B group）,siRNA transfection group（5 nmol / L group,10 nmol / L group and 20 nmol / L group）.After human colon cancer cell line SW480 were transfected by Cr-3 small interfering RNA（siRNA）,the levels of Cr-3 were determined by quantitative real-time PCR.The cell growth and invasion ability was examined by clone formation in soft agar,and Transwell,respectively.Results： Compared with Con-A group and Con-B group,siRNA transfection group（5 nmol / L group,10 nmol / L group and 20 nmol / L group） had lower levels of Cr-3 mRNA expression with lower invasion ability of colon cancer cells and in a dose-dependent manner（both P 0.05）.Conclusion： Cr-3 might play an important role in the development of human colon cancer,and down-regulation of Cr-3 by its siRNA could inhibit the invasion of human colon cancer cells.

关 键 词：大肠癌 Cripto-3基因 小干扰RNA 侵袭 

分 类 号：R393[医药卫生—基础医学]