耐药大肠埃希菌氨基糖苷类修饰酶和16S rRNA甲基化酶基因的分析  

Analysis of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drug-resistant Escherichia coli strains

作　　者：原鸿雁[1,2] 郭兵方 尹晶平[1] 孙强[1] 高晶晶[1] 苏兆亮[1] 王胜军[1] 许化溪[1] 糜祖煌[3] 

机构地区：[1]江苏大学基础医学与医学技术学院,江苏镇江212013 [2]长治市第二人民医院输血科,山西长治046000 [3]无锡市克隆遗传技术研究所,江苏无锡214026

出　　处：《江苏大学学报（医学版）》2013年第5期419-422,431,共5页Journal of Jiangsu University：Medicine Edition

基　　金：中国博士后基金资助项目(2012M511705);江苏省博士后基金资助项目(1102129C)

摘　　要：目的:了解耐药大肠埃希菌(drug-resistant Escherichia coli,DR-ECO)氨基糖苷类修饰酶和16S rRNA甲基化酶基因的分布,探讨该菌对氨基糖苷类药物耐药的分子机制。方法:采用PCR法检测20株DR-ECO中15种氨基糖苷类修饰酶基因和7种16S rRNA甲基化酶基因,并用PCR直接全自动荧光法对阳性产物进行测序。结果:20株DR-ECO中,18株检出氨基糖苷类修饰酶基因;其中,aac(3)-Ⅱ、aac(6')-Ⅰb、ant(3″)-Ⅰ、aadA4/5和aph(3')-Ⅰ的阳性检出率分别为25%、25%、5%、65%和55%;只有1株检出rmtB型16S rRNA甲基化酶基因,阳性率为5%,其余基因型均未测出。结论:本组DR-ECO对氨基糖苷类药物的耐药主要与aadA4/5和aph(3')-Ⅰ等氨基糖苷类修饰酶基因相关,而与16S rRNA甲基化酶基因无明显关系。Objective： To analyze aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in drug-resistant Escherichia coli（DR-ECO） strains,and investigate the aminoglycoside resistance mechanisms.Methods： The expressions of aminoglycoside modifying enzyme genes and 16S rRNA methylase genes in 20 DR-ECO strains were assayed by polymerase chain reaction（PCR）;and positive PCR products were sequenced by PCR direct automated fluorescence method.Results： In the 20 DR-ECO strains,18 strains expressed aminoglycoside modifying enzyme genes,the positive rate of aac（3）-Ⅱ,aac（6＇）-Ib,ant（3″）-I,aadA4/5 and aph（3＇）-I were 25%,25%,5%,65% and 55%,respectively,only 1 strains expressed rmt-B type 16S rRNA methylase gene,the positive rate was 5%,and the rest genes expressions were not detected.Conclusion： The aadA4 /5 and aph（3＇）-I,not 16S rRNA methylase gene,were closely related with the DR-ECO aminoglycoside resistance.

关 键 词：耐药大肠埃希菌 氨基糖苷类修饰酶 16S rRNA甲基化酶 

分 类 号：R373.1[医药卫生—病原生物学]