拟松材线虫携带的可培养微生物种群研究  

Culturable epizoic microbial population carried by Bursaphelenchus mucronatus

作　　者：李姝江[1] 张兴华[1,2] 朱天辉[1] 

机构地区：[1]四川农业大学林学院,雅安625014 [2]金堂县林业园林管理局,金堂610400

出　　处：《植物保护》2013年第6期50-55,共6页Plant Protection

基　　金：四川省教育厅课题"松树死亡原因及综合治理技术研究"

摘　　要：以云南松濒临枯死木为对象,利用贝尔曼漏斗法获得植物寄生性线虫,借助形态学和PCR-RELF相结合的方法鉴定出拟松材线虫,在此基础上,对其所携带的可培养微生物进行了分离纯化和鉴定,证实拟松材线虫共携带26种细菌和4种真菌,所携带可培养微生物随着拟松材线虫人工培养代数的增加,其多样性下降,人工培养3次以后,微生物的种类从30种降到9种。未纯化线虫致病性显著高于继代培养线虫,继代培养次数越多,致病性下降越快,说明体表可培养微生物参与了拟松材线虫的致病过程。The parasitic nematodes were obtained from the moribund tree of Pinus yunnanensis by using Baermann funnel method.Bursaphelenchus mucronatus was identified based on morphology and PCR-RELF,and the culturable microorganisms were isolated,purified and identified.Twenty-six species of bacteria and 4 species of fungi were verified,and the diversity of these microorganisms declined with the increase of the artificial culture generations; the species decreased from 30 to 9 after three times of artificial culture.The pathogenicity of unpurified nematodes was significantly higher than that of the subculture nematodes.The more the culture times,the faster the pathogenicity decreased.It indicated that the culturable epizoic microorganisms were involved in the pathogenic process of B.mucronatus.

关 键 词：拟松材线虫 微生物 松树 致病性 

分 类 号：S763.16[农业科学—森林保护学]