瓜类细菌性果斑病菌对硫酸铜的敏感性检测与分析  

Sensitivity test and analysis of Acidovorax citrulli to copper sulfate

作　　者：赵文龙[1] 杨玉文[1] 王铁霖[1] 蒋洁[1] 刘慧芹[1] 赵廷昌[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2013年第6期100-105,共6页Plant Protection

基　　金：公益性行业(农业)科研专项(201003066);国家西甜瓜产业技术体系(CARS-26)

摘　　要：果斑病菌两个亚群的143个菌株继代培养10代后,通过平板画线法对各菌株的硫酸铜敏感性检测结果表明：在硫酸铜浓度为300μg/mL（1.88 mmol/L）时只有一株菌株表现敏感,在浓度达到750 μg/mL（4.69 mmol/L）时所有菌株均表现敏感;浓度为450～650μg/mL （2.81～4.06 mmol/L）时分离自甜瓜的敏感菌株所占比率显著低于西瓜菌株;继代培养后所有菌株的硫酸铜敏感性没有改变,证实果斑病菌具有稳定的抗铜性.The 143 strains of Acidovorax citrulli from two subgroups were cultivated for ten generations and tested for copper sulfate sensitivity using streak plate method.The results indicated that only one strain was sensitive to copper sulfate at the concentration of 300 μg/mL （1.88 mmol/L）.All strains were sensitive at the concentration of 750 μg/mL （4.69 mmol/L）.The number of watermelon sensitive strains was much more than that of the melon strains at the concentration between 450 and 650 μg/mL （2.81-4.06 mmol/L）.The same sensitivity of subcultured strains and original strains indicated that A.citrulli has stable resistant ability to copper.

关 键 词：细菌性果斑病菌 硫酸铜 敏感性 

分 类 号：S481.4[农业科学—农药学]