济南战区陆勤干部血管紧张素转换酶基因多态性分析  

Analysis on the situation of angiotensin-converting enzyme gene polymorphism in military service officers of Jinan theater

作　　者：王菁[1] 丁茗敏[1] 张冬梅[1] 于丽华[1] 

机构地区：[1]济南军区青岛第一疗养院检验科,山东青岛266071

出　　处：《重庆医学》2013年第36期4385-4386,4389,共3页Chongqing medicine

基　　金：济南军区立项课题(JN11L067)

摘　　要：目的对济南战区陆勤干部血管紧张素转换酶(ACE)基因多态性分布情况进行调查分析。方法采用罗氏Cycler480II型荧光定量PCR分析仪进行基因型判断。同时随机抽样进行琼脂糖凝胶电泳分型进行对比分析。结果济南战区陆勤干部ACE基因DD型占23.3%,ID型占43.2%,II型占33.5%,D和I等位基因频率分别为0.44和0.56;济南战区陆勤干部女性ACE基因II型频率最高,而男性ACE基因ID型频率最高,差异具有统计学意义(P<0.05);男性与女性等位基因频率差异无统计学意义(P>0.05)。结论济南战区陆勤干部ACE基因多态性以II型和I型等位基因占优势;进行ACE基因调查对于早期发现和及时预防某些相关疾病有十分重要的意义。Objective To investigate and analysis the situation of angiotensin-converting enzyme （ACE） gene polymorphism in military service officers of Jinan theater .Methods Roche Cycler480II fluorescence quantitative PCR analyzer was used to detected ACE genotype .Meanwhile ,some samples were randomly analyzed by agarose gel electrophoresis .Results ACE DD genotype ac-counted for 23 .3% in the military service officers of Jinan theater cadres ,ID type accounted for 43 .2% ,II type accounted for 33 .5% ,D and I allele frequencies were 0 .44 and 0 .56 ,respectively ;type II ACE gene frequency was highest in female cadres ,while frequency of ID type was highest in male cadres ,the difference was statistically significant between two groups （P〈 0 .05）;the difference of allele frequency between men and women was not statistically significant （P〉0 .05） .Conclusion ACE gene polymor-phism with type II and Iallele were dominant in the military service officers of Jinan theater cadres ;ACE gene polymorphism survey is important for early detection and timely prevention of certain related diseases .

关 键 词：陆勤干部 肽基二肽酶A 多态现象 遗传 基因 

分 类 号：R82[医药卫生—临床医学]