Different Techniques of Screening Differentially Expressed Genes and Their Applying in Tea Plant  

作　　者：Xun-Lei Wang Jian-Liang Lu Yue-Rong Liang 

机构地区：[1]Zhejiang University Tea Research Institute

出　　处：《茶叶》2013年第4期223-230,共8页Journal of Tea

基　　金：financially supported by the National Science Foundation of China (Project No.31170643)

摘　　要：The genome of common varieties of tea plant,with estimated size 4000 Mb,is not clearly yet.In order to understand the gene expression difference during certain biological process,we can use transcriptome which reflects the gene expression level and is more closely related to current status instead of genome.This paper reviews several widely used techniques to screen differentially expressed genes in transcriptome level by comparing the characteristics of the methods and their application in tea plant research.The genome of common varieties of tea plant,with estimated size 4000 Mb,is not clearly yet.In order to understand the gene expression difference during certain biological process,we can use transcriptome which reflects the gene expression level and is more closely related to current status instead of genome.This paper reviews several widely used techniques to screen differentially expressed genes in transcriptome level by comparing the characteristics of the methods and their application in tea plant research.

关 键 词：差异表达基因 茶树 筛选 技术 基因表达差异 转录水平 生物过程 基因组 

分 类 号：TQ914.1[化学工程]