铬革屑酶解工艺条件的研究  

Study of Enzymolysis Conditions for Chrome Leather Shavings

作　　者：杜山山[1] 朱晓磊[1] 杜斌斌[1] 辛志荣[1] 

机构地区：[1]烟台大学化学化工学院,山东烟台264005

出　　处：《西部皮革》2013年第24期29-32,共4页West Leather

摘　　要：通过对蛋白酶产生菌进行紫外诱变,得到产生更高活性蛋白酶的菌种,然后利用此菌种产生的蛋白酶对铬革革屑进行降解实验,通过控制变量法研究了影响水解程度的体系pH值、温度、酶用量和反应时间等因素,获得高活性蛋白酶降解铬革革屑的最佳条件为:酶用量为70%,温度50℃,pH值4.0,时间2.5 h。The protease producing strain was obtained by UV mutagenesis which could generate high ac- tivity protease, which was used to hydrolyze leather shavings. It was obtained that the optimal enzymolysis conditions which enzyme amount was 70%, hydrolysis temperature was 50 ℃, pH value was 4.0 and hy- drolysis time was 2.5 h.

关 键 词：铬革革屑 降解 高活性蛋白酶 控制变量法 

分 类 号：TS59[轻工技术与工程—皮革化学与工程]