硫化氢对肝硬化大鼠肝脏缺血再灌注后TNF-α和IL-1β表达的影响  

Influence of Hysulfide on the Expression of TNF-αand IL-1βin Rats with Liver Cirrhosis after He-patic Ischemia Reperfusion

作　　者：罗锋[1] 魏来[1] 陈文雁[1] 牟燕[1] 孔高茵[1] 

机构地区：[1]湖南省人民医院麻醉科,湖南长沙410005

出　　处：《医学临床研究》2013年第11期2112-2113,2117,共3页Journal of Clinical Research

摘　　要：[目的]探讨硫氢化钠（NaHS）预处理对肝硬化大鼠肝脏缺血再灌注后肝功能变化的影响及对肝组织肿瘤坏死因子α（T N F-α）和白介素-1β（IL-1β）表达的影响。[方法]30只肝硬化大鼠随机分为3组（n＝10），包括假手术组（Sham组），肝脏缺血再灌注损伤组（HIRI组）和NaHS预处理组（NaHS组）。再灌注末取腔静脉血检测肝功能指标：谷丙转氨酶（ALT）和谷草转氨酶（AST）浓度。取部分肝组织酶联免疫吸附法（ELISA）检测 TNF-α和IL-1β表达情况。[结果]再灌注末，HIRI组、NaHS组 ALT、AST、TNF-α和IL-1β表达明显高于Sham组， NaHS组低于 HIRI组，且差异均有显著性（ P ＜0．05）。[结论]NaHS预处理能提高肝硬化大鼠抵抗肝脏缺血再灌注损伤的能力，其机制可能是通过抑制炎症反应实现。[Objective]To explore the influence of sodium hydrosulfide （NaHS ,a donor of hydrogen sulfide） precondi-tioning on the changes of liver function and the expression of tissue tumor necrosis factor-alpha（TNF-α） and interleukin-1beta （IL-1β） in rats with liver cirrhosis after hepatic ischemia reperfusion .[Methods]Thirty rats with liver cirrhosis were random-ly divided into three groups including sham group ,hepatic ischemia reperfusion injury group （HIRI group） and NaHS precon-ditioning group（NaHS group） .The inferior vena cava blood were gathered at the end of reperfusion for detecting liver func-tionindexessuchasalaninetransaminase（ALT）andaspartateaminotransferase（AST）.Theexpressionof TNF-αandIL-1βin partial liver tissues was examined by using enzyme-linked immunosorbent assay （ELISA） .[Results]At the end of reperfu-sion ,the levels of ALT and AST and the expression of TNF-αand IL-1βin HIRI group and NaHS group were obviously higher than those in sham group ,but those in NaHS group were lower than those in HIRI group ,and there were significant differences（ P＆lt;0 .05） .[Conclusion]NaHS preconditioning can increase the ability of anti-hepatic ischemia reperfusion injury of rats with liver cirrhosis .Moreover ,the effect may be achieved by inhibiting inflammatory response .

关 键 词：肝硬化  实验性 再灌注损伤 硫化氢 肿瘤坏死因子Α 白细胞介素1Β 大鼠 

分 类 号：R657.31[医药卫生—外科学]