拟南芥抗旱突变体csm1-1的鉴定及其基因克隆  

Identification of Arabidopsis drought-tolerant csm1-1 mutant and its gene cloning

作　　者：柏晓娅 顾菊 陈光朗[1] 孙成磊[1] 阳立波 曹树青[1] 

机构地区：[1]合肥工业大学生物与食品工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2013年第12期1518-1522,共5页Journal of Hefei University of Technology：Natural Science

基　　金：高等学校博士学科点专项科研基金资助项目(20120111110009);中国博士后基金资助项目(2012M521213)

摘　　要：干旱是植物生长和发育的限制因素之一,因此抗旱基因克隆和功能研究具有重要的理论意义和应用价值。文章利用甘露醇模拟干旱胁迫,以发芽为筛选指标,从拟南芥突变体库中筛选鉴定了抗旱突变体csm1-1。生理生化分析表明,csm1-1抗旱性与其电导率、可溶性糖质量比和脯氨酸积累有关。利用TAILPCR技术和RT-PCR分析发现,CSM1基因(RCI3)编码一个过氧化物酶。研究结果为进一步研究该基因调控植物抗旱的机理提供了基础。Drought is one of the limiting factors for plant growth and development, and therefore the cloning of novel drought-tolerant genes is very necessary. The wild-type and mutant lines of Arabidopsis were treated with mannitol to simulate drought stress to screen for drought-resistant mu- tants by germination analysis. One drought-tolerant csrnl-1 mutant was isolated. The results of physi- ological and biochemical analysis showed that the enhanced drought tolerance of csml-1 was related with lower conductivity and increased accumulation of soluble sugars and proline. By using TAIL-PCR technology and RT-PCR analysis, it was found that CSM1 was the RCI3 gene encoding a peroxidase. These results provide a basis for further research on the mechanism of plant drought stress responses.

关 键 词：抗旱 拟南芥 CSM1基因 鉴定TAIL-PCR技术 

分 类 号：Q754[生物学—分子生物学]