新藤黄酸对人表皮癌细胞A431增殖和凋亡的影响  

Effects of Gambogenic Acid on Proliferation and Apoptosis of Human Epidermoid Carcinoma A431 Cells

作　　者：孙美灵 程卉[1] 苏婧婧[1] 李庆林[1] 

机构地区：[1]省部共建新安医学教育部重点实验室安徽中医药大学科研实验中心,安徽合肥230038

出　　处：《安徽中医学院学报》2013年第6期67-70,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金项目(81173600);安徽省自然科学基金项目(11040606M190)

摘　　要：目的 观察新藤黄酸（gambogenic acid,GNA）对人表皮癌细胞A431增殖和凋亡的影响.方法 以体外培养的人表皮癌A431细胞株为研究对象,采用甲基噻唑基四唑检测GNA对A431细胞增殖活性的影响;倒置显微镜下观察GNA对A431细胞株细胞形态的影响;荧光显微镜下观察GNA对Hoechst 33342染色的A431细胞凋亡的影响;采用流式细胞仪检测膜联蛋白Ⅴ-异硫氰酸荧光素/碘化丙碇双染的A431细胞的凋亡率.结果 随着GNA剂量的增大,GNA对A431细胞增殖活性的抑制作用增强.倒置显微镜和荧光显微镜下观察显示,GNA作用的A431细胞形态发生明显的变化并出现显著的凋亡特征.流式细胞仪检测显示A431细胞凋亡率随着GNA作用剂量的增大而升高.结论 0.75～12.0 μmol/L GNA能够剂量依赖性地抑制A431的增殖.Objective To investigate the effects of gambogenic acid （GNA） on the proliferation and apop- tosis of human epidermoid carcinoma A431 cells. Methods The effect of GNA on the proliferative activity of A431 cells cultured in vitro was evaluated by methyl thiazolyl tetrazolium （MTT） assay; the effect of GNA on the cellular morphology of A431 cells was observed under an inverted microscope; the effect of GNA on the apoptosis of A431 cells dyed with Hoechst 33342 was observed under a fluorescence micro- scope; the apoptosis rate of A431 cells treated by Annexin g-fluorescein isothiocyanate/propidium iodide staining was determined by flow cytometry. Results GNA on the proliferative activity of A431 cells was The MTT assay showed that the inhibitory effect of enhanced as the dose of GNA increased. The A431 ceils treated with GNA showed significant changes in cellular morphology and apoptotic characteristics un- der the inverted microscope and fluorescence microscope. The flow cytometry showed that the apoptosis rate of A431 cells rose as the dose of GNA increased. Conclusion Within a dose range of 0. 75 12.0μmol/L, GNA can inhibit the proliferation of human epidermoid carcinoma A431 cells in a dose-dependent manner.

关 键 词：新藤黄酸 人表皮癌A431细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]