自制葡聚糖凝胶微型柱测定新藤黄酸聚乳酸-羟基乙酸共聚物纳米微球包封率  

Determination of Encapsulation Efficiency of Gambogenic Acid-PLGA Nanospheres Using Self-assembled Sephadex Mini-column

作　　者：李静 姚亮[1] 陈伟[1] 宋佳伟[2] 陈卫东[1] 

机构地区：[1]安徽中医药大学药学院药物代谢动力学教研室,安徽合肥230031 [2]蚌埠医学院药学系,安徽蚌埠233000

出　　处：《安徽中医学院学报》2013年第6期77-80,共4页Journal of Anhui Traditional Chinese Medical College

基　　金：安徽高校省级自然科学研究重点项目(KJ2011A190)

摘　　要：目的自制葡聚糖凝胶微型柱,建立分离测定新藤黄酸(gambogenic acid,GNA)-聚乳酸-羟基乙酸共聚物(poly lactic-co-glycolic acid,PLGA)纳米微球包封率的方法。方法采用自制葡聚糖凝胶微型柱分离PLGA纳米微球与游离药物,用浊度法判断葡聚糖凝胶微型柱对PLGA纳米微球的吸附程度;用高效液相色谱法测定PLGA纳米微球中GNA含量,并计算包封率。结果自制葡聚糖微型柱可以实现游离药物与PLGA纳米微球的分离;所测3批PLGA纳米微球的包封率平均值为82.42%。结论自制葡聚糖凝胶微型柱法简便,结果较精准,适合于GNA-PLGA纳米微球包封率的测定。Objective To establish a method for determining the encapsulation efficiency of gambogenic acid （GNA）-poly （lactic-co-glycolic acid） （PLGA） nanospheres using a self-assembled Sephadex mini col- umn. Methods Free drug was separated from PLGA nanospheres using a self-assembled Sephadex mini column, and then the adsorption of PLGA nanospheres on the Sephadex mini-column was evaluated by nephelometric analysis; the content of GNA in PLGA nanospheres was determined by high-performance liquid chromatography, and the encapsulation efficiency was calculated. Results The self assembled Sephadex mini-column enabled the separation of free drug from PLGA nanospheres; the mean encapsula- tion efficiency of three samples of PLGA nanospheres was 82.42%. Conclusion The established method is simple and accurate and can be used for determining the encapsulation efficiency of GNA-PLGA nanospheres.

关 键 词：葡聚糖凝胶微型柱 微柱离心法 新藤黄酸 纳米微球 包封率 

分 类 号：R284.2[医药卫生—中药学]