梯度洗脱反相高效液相色谱法测定粉萆薢与绵萆薢中薯蓣皂苷含量  

Gradient Elution Reversed-phase High-performance Liquid Chromatography for Determination of Dioscin in Dioscorea hypoglauca Palibin.and Dioscorea septemloba Thunb.

作　　者：刘丛彬[1] 王举涛[1] 刘劲松[1] 陈光亮[1] 

机构地区：[1]安徽中医药大学药学院现代中药安徽省重点实验室,安徽合肥230031

出　　处：《安徽中医学院学报》2013年第6期81-83,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金项目(81073112);安徽中医学院自然科学基金项目(2012zr021)

摘　　要：目的 建立粉萆薢和绵萆薢中薯蓣皂苷含量的高效液相色谱测定方法.方法 色谱柱：Gemini C18（250 mm×4.60 mm,5 μm）;流动相：乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长：203 nm;流速：0.8 ml/min;柱温：30 ℃.结果 薯蓣皂苷与其他成分分离良好,色谱峰面积与浓度呈良好的线性关系,线性范围为7.8～156.0 μg/ml（r=0.999 4）,平均回收率分别为97.99%、97.82%.结论 梯度洗脱反相高效液相色谱法准确、简便、重现性好,可用于萆薢类药材的质量评价.Objective To establish a method for determining dioscin in Dioscorea hypoglauca Palibin. and Dioscorea septemloba Thunb. by gradient elution reversed-phase high-performance liquid chromatography （RP-HPLC）. Methods RP-HPLC was performed on a Gemini C18 column （250 mm ×4.60 mm, 5 μm） with a mobile phase of acetonitrile-0.1 % phosphoric acid for gradient elution at a detection wavelength of 203 nm, a flow rate of 0.8 ml/min, and a column temperature of 30℃ Results Dioscin was well separa- ted from other constituents. The concentration of dioscin showed a good linear relationship with peak area within 7.8 - 156.0μg/ml （r=0. 999 4）. The average Palibin. and Dioscorea septemloba Thunb. were 97. recovery rates of dioscin in Dioscorea hypoglauca 99% and 97. 82%, respectively. Conclusion This RP-HPLC method is accurate, simple, and repeatable and can be used for the quality evaluation of Dioscorea.

关 键 词：萆薢 薯蓣皂苷 反相高效液相色谱法 梯度洗脱 

分 类 号：R927.2[医药卫生—药学]