高效液相色谱法同时测定植烟土壤和水中3种除草剂残留量  

Simultaneous Determination of Three Kinds of Herbicide Residues in Soil and Water in Tobacco Planting Field

作　　者：丁春霞[1,2,3] 龚道新[2,3] 肖浩[2] 任颖俊[2] 杨丽华[2] 

机构地区：[1]湖南农业大学理学院,长沙410128 [2]湖南农业大学资源环境学院,长沙410128 [3]湖南农业大学烟草研究院,长沙410128

出　　处：《中国烟草科学》2013年第5期95-99,共5页Chinese Tobacco Science

摘　　要：为了评价植烟土壤中常用3种除草剂对生态环境的安全性，建立了同时测定植烟土壤和水中高效氟吡甲禾灵、精吡氟禾草灵和精喹禾灵3种除草剂残留量的高效液相色谱分析方法。水中3种农药残留用二氯甲烷-丙酮（20：1，v：v）混合液提取，土壤中农药残留用丙酮提取，经弗罗里硅土净化后，采用HPLC法外标法定量。结果表明，3种除草剂在0.0050～5.0 mg/L范围内线性良好，相关系数均在0.99以上；土壤中添加水平在0.050～1.0 mg/kg时，3种除草剂在土壤中平均添加回收率为77.5%～110.3%，相对偏差为1.6%～10.2%，当水中添加水平在0.0050～1.0 mg/L时，回收率为88.9%～101.2%，相对偏差为0.9%～8.2%，符合农药残留分析方法要求。In order to evaluate the bio-environmental safety of three kinds of herbicides, which were often applied to tobacco soil, an analytical method was established to simultaneously determine the residues of haloxyfop-p-methyl, fluazifop-p-butyl and quizafop-p-ethyl in soil and water in tobacco planting field by high performance liquid chromatography. The residues in water was extracted with mixed solution of methylene chloride-acetone （20：1, v：v）. The soil was extracted with acetone and the extract was cleaned up by a Florisil chromatography column. The quantification was performed by external standard. The linearity was satisfied （r〉0.99） in concentration range of 0.0050-5.0 mg/L. The average recoveries of this three herbicides varied from 77.5%to 110.3%with relative standard deviation of 1.6%-10.2%, when three kinds of herbicides were spiked at 0.050-1.0 mg/kg level in soil. The average recoveries of the three herbicides varied from 88.9%to 101.2%, with relative standard deviation of 0.9%-8.2%, when three kinds of herbicides were spiked at 0.0050-1.0 mg/L level in water. The method in this study could meet the requirements of pesticide residue analysis criteria.

关 键 词：高效氟吡甲禾灵 精吡氟禾草灵 精喹禾灵 高效液相色谱 残留 

分 类 号：S481.8[农业科学—农药学]