一株拟杆菌的分离鉴定及代谢特征分析  

Isolation and Characterization of a Bacteroides Strain from Tideland of East China Sea

作　　者：刘全全[1] 郑经堂[1] 刘海昌[2] 

机构地区：[1]中国石油大学(华东),山东青岛266580 [2]农业部沼气科学研究所,成都610041

出　　处：《中国沼气》2013年第6期3-8,共6页China Biogas

摘　　要：纤维素在地球上分布广泛，其降解过程是自然界碳素循环的中心环节。从东海潮间带分离出一株纤维素降解菌D-2，该菌株严格厌氧菌，呈不规则的球杆状，单个或成簇出现，革兰氏阴性，无鞭毛，不运动。直径约1～3μm。最适生长温度37℃，最适盐浓度20g·L^-1，最适pH为7．0，酵母膏刺激生长。菌株D-2与模式菌株Bacteroides reticulotermitis JCM 10512^T的16SrRNA基因序列相似性为99％。Cellulose is most widely distributed on earth, and its degradation is central to a natural carbon cycle. A Bacteroides reticulotermitis, strain D-2 ,was isolated from tideland soil of the East China Sea, 0.5 μm × 1 -3μm , obligate anaerobe, occurred in clusters of two to four cells. Strain D-2 grew fastest at 37℃, pH 7.0 ,NaC1 20 g × L^-l ,and with yeast extract. Sequence analysis of 16S rRNA genes showed that it revealed similarities of 99% with Bacteroides reticulotermitis JCM 105127.

关 键 词：厌氧纤维素降解 纤维素 潮间带 

分 类 号：S216.4[农业科学—农业机械化工程]